Differentiation, phenotype, and function of interleukin-17–producing human Vγ9Vδ2 T cells by Nadia Caccamo, Carmela La Mendola, Valentina Orlando, Serena.

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Differentiation, phenotype, and function of interleukin-17–producing human Vγ9Vδ2 T cells by Nadia Caccamo, Carmela La Mendola, Valentina Orlando, Serena Meraviglia, Matilde Todaro, Giorgio Stassi, Guido Sireci, Jean Jacques Fournié, and Francesco Dieli Blood Volume 118(1):129-138 July 7, 2011 ©2011 by American Society of Hematology

Polarization of IL-17+ Vγ9Vδ2 T cells is induced by antigen and IL-1β, IL-6, IL-23, and TGF-β. Polarization of IL-17+ Vγ9Vδ2 T cells is induced by antigen and IL-1β, IL-6, IL-23, and TGF-β. Intracellular cytokine staining for IL-17 and IFN-γ in naive (CD45RA+CD27+) human Vγ9Vδ2 T cells primed for 6 days with an equal number of irradiated DCs and IPP, in the presence of various combinations of cytokines, then incubated for 6-7 days more in IL-2 and stimulated for 6 hours with IPP. (A) Cumulative results (mean values ± SD) of 3 different experiments each performed with 5 different healthy donors. *P < .01 and **P < .05 compared with the medium group. (B) Typical flow cytometry panels of a representative experiment. Numbers in quadrants indicate percent cells in each. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology

Priming with antigen and IL-1β, IL-6, IL-23, and TGF-β induces exclusive IL-17 production and RORC expression in differentiating Vγ9Vδ2 T cells. Priming with antigen and IL-1β, IL-6, IL-23, and TGF-β induces exclusive IL-17 production and RORC expression in differentiating Vγ9Vδ2 T cells. (A) Intracellular cytokine staining for IL-17, IFN-γ, IL-22, IL-4, and IL-10 in naive (CD45RA+CD27+) human Vγ9Vδ2 T cells primed for 6 days with an equal number of irradiated DCs and IPP in the presence of a cocktail of 4 cytokines (IL-1β, IL-6, IL-23, and TGF-β) then incubated for 6-7 days more in IL-2 and stimulated for 6 hours with IPP. (B) RT-PCR of the expression of RORC and TBX21 in cells primed with antigen in the presence or absence (medium) of cytokines, as described in panel A. Data represent the mean values ± SD of 3 separate experiments, each performed with 2 different healthy donors. *P < .001 compared with the medium group. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology

Antigen and cytokines differently regulate expression of lineage-specifying transcription factors in IL-17–differentiating Vγ9Vδ2 T cells. Antigen and cytokines differently regulate expression of lineage-specifying transcription factors in IL-17–differentiating Vγ9Vδ2 T cells. RT-PCR of the expression of IL1βR, IL6R, TGFβR, IL23R, IL17, and RORC in naive (CD45RA+CD27+) human Vγ9Vδ2 T cells primed for various times (horizontal axes) with antigen, or left unprimed, in the presence or absence of cytokines. Data represent the mean values ± SD of 4 separate experiments, each performed with 5 different donors. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology

Surface phenotype of IL-17+ Vγ9Vδ2 T cells. Surface phenotype of IL-17+ Vγ9Vδ2 T cells. Vγ9Vδ2 T cells were primed for 6 days with an equal number of irradiated DCs and IPP in the presence of cytokines and then incubated for 6-7 days more in IL-2 and stimulated for 6 hours with IPP. After intracellular staining for IL-17 and IFN-γ, cells were surface stained for several different markers. (A) Chemokine receptor expression is shown on gating on IL-17+ or IFN-γ+ Vγ9Vδ2 T cells. The vertical line in each panel indicates the negative cutoff as determined by staining with isotype-control mAbs. (B) Surface markers expression on IL-17+ Vγ9Vδ2 T cells (violet-filled lines). Green open lines indicate staining with isotype-control mAbs. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology

IL-17+ Vγ9Vδ2 T cells promote CXCL8-mediated neutrophil migration and phagocytic activity. IL-17+ Vγ9Vδ2 T cells promote CXCL8-mediated neutrophil migration and phagocytic activity. (A) Neutrophils were placed in the top well of a Boyden Chamber, and IL17+ Vγ9Vδ2 T cells were placed in the lower chamber in the presence or absence of IPP. In some experiments, neutralizing mAb to CXCL8 or isotype control mAbs were added to the lower chamber. After 3 hours of incubation at 37°C, migrated neutrophils adherent to the lower side of the membrane were stained and counted. Data are expressed as percentage of migrated cells among input. (B) Neutrophils were incubated with PE-fluorescent beads in the presence of IL17+ Vγ9Vδ2 T cells and IPP. In some experiments, neutralizing mAbs to CXCL8 or isotype control mAbs were added to cultures. After 2 hours, the percentage of PE+ neutrophils was determined FACS. Data are expressed as percentage PE+ neutrophils. *P < .01 and **P < .02 compared with all other groups. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology

IL-17+ Vγ9Vδ2 T cells promote IL-17–dependent production of β-defensin by epithelial cells. IL-17+ Vγ9Vδ2 T cells promote IL-17–dependent production of β-defensin by epithelial cells. HT29 epithelial cells were incubated with IL-17+ Vγ9Vδ2 T cells in the presence or absence of IPP. In some experiments, neutralizing mAbs to IL-17 or isotype control mAbs were added to cultures. After 24 hours, supernatants were collected and β-defensins quantitated by commercially available ELISA. *P < .005 compared with all other groups. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology

Frequencies of IL-17+ Vγ9Vδ2 T cells in control subjects and in patients with bacterial meningitis. Frequencies of IL-17+ Vγ9Vδ2 T cells in control subjects and in patients with bacterial meningitis. (A) Frequencies of IL-17–producing cells among Vγ9Vδ2 T cells from the peripheral blood of adult healthy donors (A-HC; n = 30), control children (C-HC; n = 8), and children affected by bacterial meningitis (Men; n = 12) on short-term stimulation with antigen (IPP). (B) The frequency of IL-17+ Vγ9Vδ2 T cells in the peripheral blood of children affected by bacterial meningitis (n = 12), before and after successful therapy, determined on antigen stimulation, as above described. (C) Frequencies of IL-17+ Vγ9Vδ2 T cells in the cerebrospinal fluid (CSF) of control children (HC-CSF; n = 8) and children affected by bacterial meningitis (Men-CSF; n = 12) on short-term stimulation with antigen (IPP). *P < .001 compared with all other groups. Nadia Caccamo et al. Blood 2011;118:129-138 ©2011 by American Society of Hematology