Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve Simon P Hoerstrup, MD, Gregor Zünd, MD, Andreina.

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Presentation transcript:

Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve Simon P Hoerstrup, MD, Gregor Zünd, MD, Andreina Schoeberlein, PhD, Qing Ye, MD, Paul R Vogt, MD, Marko I Turina, MD The Annals of Thoracic Surgery Volume 66, Issue 5, Pages 1653-1657 (November 1998) DOI: 10.1016/S0003-4975(98)00796-6

Fig 1 Concept of tissue engineering of a bioprosthetic heart valve. (FACS = fluorescence activated cell sorting.) The Annals of Thoracic Surgery 1998 66, 1653-1657DOI: (10.1016/S0003-4975(98)00796-6)

Fig 2 Histogram flow cytometry. (A) Negative control, unlabeled mixed cell population of human aortic tissue. (B) Positive assay, Dil-Ac-LDL–labeled mixed cell population of human aortic tissue (endothelial cells = LDL+ [fluorescent], myofibroblasts = LDL−). (C) “Gating” of significant areas for fluorescence activated cell sorting. (M3 = nonfluorescent [myofibroblasts]; M2 = fluorescent [endothelial cells]; M1 = with regard to fluorescence ambiguous cell population [safety margin for sorting procedure].) The Annals of Thoracic Surgery 1998 66, 1653-1657DOI: (10.1016/S0003-4975(98)00796-6)

Fig 3 Fluorescence microscopy: full fluorescence of the endothelial cell line (LDL+). (×100 before 35% reduction.) The Annals of Thoracic Surgery 1998 66, 1653-1657DOI: (10.1016/S0003-4975(98)00796-6)

Fig 4 Formation of endothelial monolayer. CD34 stain demonstrates a monolayer of endothelial cells (a) on the surface of a fibroblast core (b) with polymer fibers (c). The Annals of Thoracic Surgery 1998 66, 1653-1657DOI: (10.1016/S0003-4975(98)00796-6)