Laboratory Diagnosis of Clostridium difficile Infection

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Laboratory Diagnosis of Clostridium difficile Infection Fred C. Tenover, Ellen Jo Baron, Lance R. Peterson, David H. Persing The Journal of Molecular Diagnostics Volume 13, Issue 6, Pages 573-582 (November 2011) DOI: 10.1016/j.jmoldx.2011.06.001 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Amino acid heterogeneity (red) and predicted antigenicity of toxin B protein (black lines) based on an alignment of amino acid sequences of toxin B from 16 C. difficile isolates. The red-shaded plot is the variability of the amino acid sequences of toxin B at each amino acid position; great amino acid diversity is indicated by higher peaks. The black line indicates the predicted antigenicity of the protein based on the algorithm of Kolaskar and Tongaonkar (KT)52 (high peaks indicate high antigenicity). The blue bars indicate regions of DNA sequence conservation among the 16 isolates. The 16 isolates include strains of PCR ribotypes 001, 017, 027, and 078 and pulsed-field gel electrophoresis types NAP1, NAP7, and NAP8. The Journal of Molecular Diagnostics 2011 13, 573-582DOI: (10.1016/j.jmoldx.2011.06.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Steps involved in four FDA-cleared nucleic acid amplification assays for the detection of CDI. Data were extracted from package inserts and published literature.34,36,53,54 rxn, reaction. The Journal of Molecular Diagnostics 2011 13, 573-582DOI: (10.1016/j.jmoldx.2011.06.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions