Pharmacokinetics of 18F-labeled trovafloxacin in normal and Escherichia coli-infected rats and rabbits studied with positron emission tomography  Alan J. Fischman, John W. Babich, Nathaniel M. Alpert, John Vincent, Robert A. Wilkinson, Ronald J. Callahan, John A. Correia, Robert H. Rubin  Clinical Microbiology and Infection  Volume 3, Issue 1, Pages 63-72 (February 1997) DOI: 10.1111/j.1469-0691.1997.tb00253.x Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Biodistribution of trovafloxacin in normal rats (open bars) and uninvolved tissues of infected rats (solid bars) at 5, 30, 60 and 120 min after injection. The data are expressed as μg/g. The animals were infected 24 h before injection. Each value is the mean ± SEM for six animals. *p < 0.05; **p < 0.01. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Trovafloxacin concentrations (μg/g) in normal and infected rat muscle at 5, 30, 60 and 120 min after injection. The animals were infected 24 h before injection. Normal* indicates uninfected muscle of infected rats. Each value is the mean±SEM for six animals. *p < 0.05; **p < 0.01. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Sagittal and coronal PET images of a rabbit 30 min after injection of [18F]trovafloxacin plus 10 mg/kg unlabeled trovafloxacin. Drug concentrations are indicated by the color bar. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 Blood clearance of trovafloxacin in normal and infected rabbits. Results from individual animals are not indicated. Each value is the mean ± SEM for four animals. The data were well described by biexponential functions. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 5 Tissue accumulation curves of trovafloxacin in normal (n = 4) and infected (n = 4) rabbits. Since there was no significant difference in drug concentrations between uninfected tissues of normal and infected animals, these data were pooled for analysis and each value represents the mean ± SEM for eight animals. Concentrations were measured by PET. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 6 Biodistribution of trovafloxacin in normal rabbits and rabbits with deep thigh E. coli infections at 2 h after injection. The data are expressed as μg/g. The animals were infected 24 h before injection. Each value is the mean ± SEM for four animals. Concentrations were determined by direct tissue radioactivity measurements. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 7 Trovafloxacin concentrations (μg/g) in normal and infected rabbit muscle at 120 min after injection determined by direct tissue radioactivity measurements. The animals were infected 24 h before injection. Normal* indicates uninfected muscle of infected rabbits. Each value is the mean ± SEM for four animals. Clinical Microbiology and Infection 1997 3, 63-72DOI: (10.1111/j.1469-0691.1997.tb00253.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions