Chronic Pancreatitis and Pancreatic Cancer: Prediction and Mechanism Tooru Shimosegawa, Kiyoshi Kume, Kennichi Satoh  Clinical Gastroenterology and Hepatology  Volume 7, Issue 11, Pages S23-S28 (November 2009) DOI: 10.1016/j.cgh.2009.07.042 Copyright © 2009 AGA Institute Terms and Conditions

Figure 1 (A) Restriction fragment length polymorphism (RFLP) analysis of microdissected samples for K-ras mutation. Any mutation in codon 12 eliminates the restriction site so the mutated alle is resistant to digestion with BstNI. The polymerase chain reaction products from pancreatic cancer cell lines (Panc-1 and BxPC3) and microdissected carcinoma lesions were treated with BstNI. Panc-1 and BxPC3 were used as controls for mutated and wild type K-ras, respectively. K-ras mutation at codon 12 was found in Panc-1, CPPCa, and PCa, while no mutation was observed in BxPC3 cells. Expression of NF-κB p65 in (B) CPPCa and (C) PCa tissues. Cytoplasmic (arrows) and nuclear staining (arrowheads) was seen in these carcinoma tissues. Accumulation of p53 in (D) PCa and (E) CPPCa tissues. Intense nuclear expression of p53 (arrowheads) was observed in both types of carcinoma cells. Original magnification, ×33. Clinical Gastroenterology and Hepatology 2009 7, S23-S28DOI: (10.1016/j.cgh.2009.07.042) Copyright © 2009 AGA Institute Terms and Conditions

Figure 2 (A) Intense expression of α-SMA was seen in fibroblasts (arrowheads) surrounding mouse metaplastic duct induced by DMBA. The expression of α-SMA was also observed in vessels (arrow). Original magnification, ×66. (B) Intense expression of α-SMA was found in mouse fibroblasts surrounding poorly differentiated carcinoma cells induced by DMBA. (Original magnification, ×33). Clinical Gastroenterology and Hepatology 2009 7, S23-S28DOI: (10.1016/j.cgh.2009.07.042) Copyright © 2009 AGA Institute Terms and Conditions

Figure 3 PSC-conditioned media induced cellular growth of HPDE cells. Cell count assay revealed approximately a 2-fold increase in number of HPDE cells cultured with PSC-conditioned media compared with those cultured with control media. CM, conditioned media. Clinical Gastroenterology and Hepatology 2009 7, S23-S28DOI: (10.1016/j.cgh.2009.07.042) Copyright © 2009 AGA Institute Terms and Conditions

Figure 4 (A) Photograph showing the HPDE cells cultured with PSC-conditioned media (right panel) and those with control media (left panel) at 5 days after seeding 500 cells in each well. (B) HPDE cells cultured with PSC-conditioned media formed approximately 2-fold more colonies than those cultured with control media. CM, conditioned media. Clinical Gastroenterology and Hepatology 2009 7, S23-S28DOI: (10.1016/j.cgh.2009.07.042) Copyright © 2009 AGA Institute Terms and Conditions