Evaluation of quantitative polymerase chain reaction markers for the detection of breast cancer cells in ovarian tissue stored for fertility preservation  Laurence Bockstaele, Ph.D., Selma Boulenouar, Ph.D., Géraldine Van Den Steen, B.Sc., Julie Dechène, B.Sc., Sophie Tsepelidis, M.D., Ligia Craciun, Ph.D., Jean-Christophe Noël, M.D., Ph.D., Isabelle Demeestere, M.D., Ph.D.  Fertility and Sterility  Volume 104, Issue 2, Pages 410-417.e4 (August 2015) DOI: 10.1016/j.fertnstert.2015.04.036 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunohistologic analysis of ovarian xenotransplants after 6 months of graft. Two fragments per patient were allocated to histologic analysis after long-term xenografting. Twenty percent to 30% of each xenotransplant were analyzed by immunohistochemistry. Serial sections of ovarian xenotransplants were immunostained with CK-19, WT-1, GCDFP-15, or MGB-1 antibodies. A total of 70, 90, 84, 105, 60, and 120 sections were analyzed from cortex 11-F3, cortex 11-F4, cortex 12-R5, cortex 12-L2, cortex 13-L2, and cortex 13-G4, respectively. (A) Representative photographs of healthy primordial, primary, secondary, and antral follicles identified in ovarian xenotransplants. (B) Photographs of CK-19–positive structures identified inside one fragment of cortex 4 and cortex 12, and two fragments of cortex 13. Adjacent sections were immunostained with WT-1, GCDFP-15, and MGB-1 antibodies. NA = not available. Scale bar = 100 μm. Fertility and Sterility 2015 104, 410-417.e4DOI: (10.1016/j.fertnstert.2015.04.036) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions