Estrogen and progesterone receptor isoform distribution through the menstrual cycle in uteri with and without adenomyosis Mohamed Khairy Mehasseb, M.B.B.Ch.,

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Estrogen and progesterone receptor isoform distribution through the menstrual cycle in uteri with and without adenomyosis Mohamed Khairy Mehasseb, M.B.B.Ch., M.Sc., M.D., Ph.D., Rina Panchal, M.B.B.S., Anthony Henry Taylor, B.Sc., M.Sc., Ph.D., Laurence Brown, B.Sc., F.R.C.Path., Stephen Charles Bell, B.Sc., F.R.C.Path., Ph.D., Marwan Habiba, M.B.B.Ch., M.Sc., Ph.D. Fertility and Sterility Volume 95, Issue 7, Pages 2228-2235.e1 (June 2011) DOI: 10.1016/j.fertnstert.2011.02.051 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Histomorphometric analyses of estrogen receptor α (ER-α) staining patterns in the glands, stroma, and foci (▲) of normal (•) and adenomyotic (○) uteri. Control samples: 5 early proliferative (EP), 6 midproliferative (MP), 5 late proliferative (LP); 8 early secretory (ES), 6 midsecretory (MS), and 5 late secretory (LS). Adenomyotic samples: 10 EP, 11 MP, 6 LP; 8 ES, 9 MS, and 10 LS. Data are presented as the mean ± standard error of the mean. ∗P<.05 adenomyotic % positive cells compared with control, Student’s unpaired t-test. Fertility and Sterility 2011 95, 2228-2235.e1DOI: (10.1016/j.fertnstert.2011.02.051) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Histomorphometric analyses of estrogen receptor β (ER-β) staining patterns in the glands, stroma, and foci (▲) of normal (•) and adenomyotic (○) uteri. Data are presented as the mean ± standard error of the mean. Control samples: 5 early proliferative (EP), 6 midproliferative (MP), 5 late proliferative (LP); 8 early secretory (ES), 6 midsecretory (MS), and 5 late secretory (LS). Adenomyotic samples: 10 EP, 11 MP, 6 LP, 8 ES, 9 MS, and 10 LS. ∗P<.05 adenomyotic % positive cells compared with control, Student’s unpaired t-test. Fertility and Sterility 2011 95, 2228-2235.e1DOI: (10.1016/j.fertnstert.2011.02.051) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Histomorphometric analyses of progesterone receptor A (PR-A) staining patterns in the glands, stroma, and foci (▲) of normal (•) and adenomyotic (○) uteri. Data are presented as the mean ± standard error of the mean. Control samples: 5 early proliferative (EP), 6 midproliferative (MP), 5 late proliferative (LP); 8 early secretory (ES), 6 midsecretory (MS), and 5 late secretory (LS). Adenomyotic samples: 10 EP, 11 MP, 6 LP; 8 ES, 9 MS, and 10 LS. ∗P<.05 adenomyotic % positive cells compared with control, Student’s unpaired t-test. Fertility and Sterility 2011 95, 2228-2235.e1DOI: (10.1016/j.fertnstert.2011.02.051) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Histomorphometric analyses of progesterone receptor B (PR-B) staining patterns in the glands, stroma, and foci (▲) of normal (•) and adenomyotic (○) uteri. Data are presented as the mean ± standard error of the mean. Control samples: 5 early proliferative (EP), 6 midproliferative (MP), 5 late proliferative (LP); 8 early secretory (ES), 6 midsecretory (MS), and 5 late secretory (LS). Adenomyotic samples: 10 EP, 11 MP, 6 LP; 8 ES, 9 MS, and 10 LS. ∗P<.05 adenomyotic % positive cells compared with control, Student’s unpaired t-test. Fertility and Sterility 2011 95, 2228-2235.e1DOI: (10.1016/j.fertnstert.2011.02.051) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Comparison of progesterone receptor B (PR-B) staining in the glands and stroma of the control and adenomyotic basalis through the menstrual cycle. Photomicrographic images of PR-B staining in the early (EP), middle (MP), and late (LP) proliferative phase and early (ES), middle (MS), and late (LS) secretory phases of the menstrual cycle in control (left panels) and adenomyotic basalis (right panels) are shown. The percentage of positively stained immunoreactive cell nuclei or cytoplasm was obtained by counting the number of positive (brown-stained) and negative (blue-stained) cells in the field. The images were taken at ×400 magnification. Fertility and Sterility 2011 95, 2228-2235.e1DOI: (10.1016/j.fertnstert.2011.02.051) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions