Apolipoprotein E-containing high-density lipoprotein (HDL) modifies the impact of cholesterol-overloaded HDL on incident coronary heart disease risk: A community-based cohort study  Yue Qi, MD, PhD, Jing Liu, MD, PhD, Wei Wang, MD, Miao Wang, MD, Fan Zhao, PhD, Jiayi Sun, MD, Jun Liu, MD, Dong Zhao, MD, PhD  Journal of Clinical Lipidology  Volume 12, Issue 1, Pages 89-98.e2 (January 2018) DOI: 10.1016/j.jacl.2017.11.003 Copyright © 2017 National Lipid Association Terms and Conditions

Figure 1 Distribution of APOE-containing HDLC (A) and the ratio of APOE-containing HDLC to total HDLC (B). APOE, apolipoprotein E; APOE-HDLC, apolipoprotein E-containing high-density lipoprotein cholesterol. Journal of Clinical Lipidology 2018 12, 89-98.e2DOI: (10.1016/j.jacl.2017.11.003) Copyright © 2017 National Lipid Association Terms and Conditions

Figure 2 Percent of the APOE-HDLC/HDLC ratio subgroups among subgroups of the HDLC/HDLP ratio. APOE-HDLC/HDLC ratio, ratio of apolipoprotein E-containing high-density lipoprotein cholesterol to total high-density lipoprotein cholesterol; HDL-C/P ratio, ratio of high-density lipoprotein cholesterol to high-density lipoprotein particle numbers. Journal of Clinical Lipidology 2018 12, 89-98.e2DOI: (10.1016/j.jacl.2017.11.003) Copyright © 2017 National Lipid Association Terms and Conditions

Figure 3 Cox regression analysis of combined subgroups of the HDL-C/P ratio and APOE-HDLC/HDLC ratio on the long-term risk of coronary heart disease incidence. All substantial models are additionally adjusted for age (5 years), sex, body mass index (1 kg/m2), systolic BP (10 mm Hg), LDL cholesterol (20 mg/mL), diabetes, high triglyceride (>150 mg/dL), log-transformed hs-CRP levels, HDL size (0.4 nm), log-transformed lipoprotein-associated phospholipase A2 levels, lipid-lowering treatment, and smoking. The HDL-C/P ratio and APOE-HDLC/HDLC ratio were categorized and cross-combined into the following 4 groups: low HDL-C/P ratio (<47.5) and low APOE-HDLC/HDLC ratio (<9.8%); low HDL-C/P ratio (<47.5) and high APOE-HDLC/HDLC ratio (≥9.8%); high HDL-C/P ratio (≥47.5) and low APOE-HDLC/HDLC ratio (<9.8%); and high HDL-C/P ratio (≥47.5) and high APOE-HDLC/HDLC ratio (≥9.8%). The subgroup with a low HDL-C/P ratio and low APOE-HDLC/HDLC ratio was defined as the reference group. *P value < .050. There were 15, 1, 7, and 2 CHD incident events in the subgroups of a low HDL-C/P ratio and low APOE-HDLC/HDLC ratio, the subgroups of a low HDL-C/P ratio and high APOE-HDLC/HDLC ratio, the subgroups of a high HDL-C/P ratio and low APOE-HDLC/HDLC ratio, and the subgroups of a high HDL-C/P ratio and high APOE-HDLC/HDLC ratio, respectively. APOE-HDLC/HDLC ratio, ratio of apolipoprotein E-containing high-density lipoprotein cholesterol to total high-density lipoprotein cholesterol; BP, blood pressure; CI, confidence interval; CHD, coronary heart disease; HDL, high-density lipoprotein; HDL-C/P ratio, ratio of high-density lipoprotein cholesterol to high-density lipoprotein particle numbers; HR, hazard ratio; hs-CRP, high-sensitivity C-reactive protein; LDL, low-density lipoprotein. Journal of Clinical Lipidology 2018 12, 89-98.e2DOI: (10.1016/j.jacl.2017.11.003) Copyright © 2017 National Lipid Association Terms and Conditions

Supplementary Figure S1 Cox regression analysis of the combined subgroups of HDLC level and the APOE-HDLC/HDLC ratio on the long-term risk of coronary heart disease incidence. HDLC and the APOE-HDLC/HDLC ratio were categorized and cross-combined into the following 4 groups: low HDLC (<60 mg/dL) and low APOE-HDLC/HDLC ratio (<9.8%); low HDLC (<60 mg/dL) and high APOE-HDLC/HDLC ratio (≥9.8%); high HDLC (≥60 mg/dL) and low APOE-HDLC/HDLC ratio (<9.8%); and high HDLC (≥60 mg/dL) and high APOE-HDLC/HDLC ratio (≥9.8%). The subgroup with a low HDL-C and low APOE-HDLC/HDLC ratio was defined as the reference group. All substantial models are additionally adjusted for age (5 years), sex, body mass index (1 kg/m2), systolic BP (10 mm Hg), LDL cholesterol (20 mg/mL), diabetes, high triglyceride (>150 mg/dL), log-transformed hs-CRP levels, HDL size (0.4 nm), log-transformed lipoprotein-associated phospholipase A2 levels, lipid-lowering treatment, and smoking. APOE-HDLC/HDLC ratio, the ratio of apolipoprotein E-containing high-density lipoprotein cholesterol to total high-density lipoprotein cholesterol; BP, blood pressure; CHD, coronary heart disease; CI, confidence interval; HDLC, high-density lipoprotein cholesterol; HR, hazard ratio; hs-CRP, high-sensitivity C-reactive protein; LDL, low-density lipoprotein. Journal of Clinical Lipidology 2018 12, 89-98.e2DOI: (10.1016/j.jacl.2017.11.003) Copyright © 2017 National Lipid Association Terms and Conditions