Lesson 1.4: Microscopes.

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Presentation transcript:

Lesson 1.4: Microscopes

Types of Microscopes: The compound light microscope contains two types of lenses that magnify an object Ocular lens: closest to the eye Objective lens: closest to the object

Diaphragm: controls the amount of light coming through the microscope Nosepiece: contains the 3 revolving objective lenses Adjustment Knobs: adjust clarity of the object Coarse- find Fine- focus Other Parts: Body Tube Arm Base Stage/Stage Clips Light

Example: 10 * 40 = 400x (400 times zoomed in) To calculate the microscope’s power of magnification, multiply the power of the ocular lens (always 10x) by the power of the objective lens (4x, 10x, or 40x) Example: 10 * 40 = 400x (400 times zoomed in)

Focusing a Specimen: Use the Nosepiece to set MS to 4x Place the slide on the stage using the stage clips Center the specimen using the mechanical stage knobs Use the mechanical stage knobs to raise the stage Focus the specimen (using the fine & coarse adjustment knobs) Use the Nosepiece to set MS to 10x Focus the specimen again Use the Nosepiece to set MS to 40x Use the Nosepiece to set MS back to 4x before you swap slides and/or turn the MS off

DO NOT TAKE HOME, THANKS! Types of Microscopes: A dissecting microscope is used to see 3D, not 2D like a compound microscope They have a lower magnification than a compound microscope Used to observe and dissect small organisms

Types of Microscopes: The electron microscope has the highest magnification and resolution possible (Up to 50,000,000x) Uses electrons to produce images of small, even subatomic particles

Scanning Electron Microscope (SEM) Electrons scan the object to create a 3-D image of the surface of the object

Transmission Electron Microscope (TEM) A focused electron beam (like a laser), is used to look inside specimen (like an x-ray) This is the MOST powerful microscope!