Comparison between conventional wide-field detection and multifocus detection. Comparison between conventional wide-field detection and multifocus detection.

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Comparison between conventional wide-field detection and multifocus detection. Comparison between conventional wide-field detection and multifocus detection. (A and B) The whole volume is excited in the wide-field configuration, and fluorescence from out-of-focus molecules constitutes the background of the recorded in-focus signal. (C and D) In the multifocus configuration, the 3D extent of the PSF is used to localize molecules within the volume as information is obtained from multiple focal planes. Bassam Hajj et al. PNAS 2014;111:49:17480-17485 ©2014 by National Academy of Sciences