Neurotransmitter Recovery and Degradation Active re-uptake is very important in the CNS

Principles of Synaptic Integration Integration of EPSPs Contribution of dendritic properties: the length constant Inhibition Modulation

Studying Neurotransmitter Systems Criteria for Neurotransmitter Status Molecule must be synthesized and stored in presynaptic neuron Molecule must be released by the presynaptic terminal upon stimulation Molecule must elicit response that mimics that caused by release from the presynaptic neuron

Molecule must be synthesized and stored in presynaptic neuron Immunocytochemistry In Situ Hybridization Autoradiography

Molecule must be released by the presynaptic terminal upon stimulation Chemistry: Stimulate until you release enough transmitter to complete a chemical analysis Physiology: Use a bioassay

Ionophoresis & "puffing" Molecule must elicit response that mimics that caused by synaptic release Ionophoresis & "puffing"

Studying Receptors Ligand binding methods Molecular analysis

Neurotransmitter Chemistry Chemical categories Cholinergic Catecholaminergic Serotonergic Amino Acidergic Peptidergic

Transmitter Gated Channels AChR (we've seen it) Amino acid-gated Glutamate Gaba Glycine