Volume 121, Issue 6, Pages 1275-1280 (December 2001) WNT1 inducible signaling pathway protein 3, WISP-3, a novel target gene in colorectal carcinomas with microsatellite instability  Lin Thorstensen, Chieu B. Diep, Gunn I. Meling, Trude H. Aagesen, Christian H. Ahrens, Torleiv O. Rognum, Ragnhild A. Lothe  Gastroenterology  Volume 121, Issue 6, Pages 1275-1280 (December 2001) DOI: 10.1053/gast.2001.29570 Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 1 Frequency of frameshift mutations in 11 genes in primary colorectal carcinomas with MSI. (Blue bar), results of the present study; and (white bar), mean mutation frequency evaluated from previous reports. *Target genes investigated by several groups and the mutation frequency range were: TGFβRII, 63%–93%26,35-39; BAX, 31%–67%10,11,35,36,38-40; hMSH3, 29%–64%11,35,36,38,39,41; and hMSH6, 12%–50%.11,35,36,38,39,41 Gastroenterology 2001 121, 1275-1280DOI: (10.1053/gast.2001.29570) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 2 Frameshift mutations in a novel downstream target gene, WISP-3, in colorectal carcinomas with MSI. To the left, the PCR fragment analyses on an ABI PRISM 310 Analyzer are shown (the numbers in the blue boxes indicate the size of the PCR products). Sequenced PCR products of the normal sample and 1 tumor (C1314) are shown to the right. Tumors C1141 and C1314 reveal mutations in WISP-3. wt, wild-type; mut, mutation. Gastroenterology 2001 121, 1275-1280DOI: (10.1053/gast.2001.29570) Copyright © 2001 American Gastroenterological Association Terms and Conditions