Figure e-2 A B C endo TDP-43 Wild-type Ran Merge+DAPI D E F

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Figure e-2 A B C endo TDP-43 Wild-type Ran Merge+DAPI D E F Figure e-2. Nuclear retention of TDP-43 and FUS depend on Ran-GTPase activity. HeLa cells were transfected with wild-type (A-C and G-I) or dominant-negative Q69L Ran-GTPase tagged with Flag (D-F and J-L), and cells were processed for double immunofluorescence with anti-TDP-43 (Proteintech; green) (A-F), anti-N-terminal FUS (Bethyl Labs; green) (G-L), and anti-Flag (Sigma; red) antibodies. Note that Q69L Ran-GTPase displaces endogenous TDP-43 and FUS from the nucleus to the cytoplasm (arrows). Scale bar = 10 μm. endo TDP-43 Q69L Ran Merge+DAPI G H I endo Fus Wild-type Ran Merge+DAPI J K L endoFus Q69L Ran Merge+DAPI