Validation of Tn-seq fitness defects in rich and defined media.

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Validation of Tn-seq fitness defects in rich and defined media. Validation of Tn-seq fitness defects in rich and defined media. (A) Tn-seq results (rich and defined media) for 13 genes (observed versus expected Tn insertion reads) displayed as a bar graph. Values less than 1 are predictive of a gene that is required for optimal fitness in that environment. (B) Bar graph depicting the fitness of mutants compared to the fitness of the wild type using a 1 × 1 competition method. A competition index of >1 indicates that the mutant grew better than the wild type did. A competition index of <1 indicates that the mutant displayed reduced fitness compared to the wild-type strain. The genes and proteins they encode follow: sufC, ABC transporter involved in Fe-S cluster assembly; rpmH, 50S ribosomal protein L34; pppL, phosphoprotein phosphatase; pknB, serine/threonine phosphatase; divIB, cell division protein; ltaS, phosphoglycerol transferase; rgpI, glycosyltransferase 2-like protein; SMu.852, transcriptional regulator; vicK, histidine kinase; SMu.1542, diacylglycerol kinase; clpP, ATP-dependent Clp protease; dltA, d-alanine-d-alanyl carrier protein ligase; scrB, sucrose-6-phosphate hydrolase. *, P < 0.05; **, P < 0.01. Robert C. Shields et al. mSphere 2018; doi:10.1128/mSphere.00031-18