Cardioplegia prevents ischemia-induced transcriptional alterations of cytoprotective genes in rat hearts: A DNA microarray study Steve J. Schomisch,

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Cardioplegia prevents ischemia-induced transcriptional alterations of cytoprotective genes in rat hearts: A DNA microarray study Steve J. Schomisch, BS, Deborah G. Murdock, PhD, Nasim Hedayati, MD, Joseph L. Carino, BS, Edward J. Lesnefsky, MD, Brian L. Cmolik, MD The Journal of Thoracic and Cardiovascular Surgery Volume 130, Issue 4, Pages 1151.e1-1151.e13 (October 2005) DOI: 10.1016/j.jtcvs.2005.06.027 Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Recovery of function (top) at 180 minutes and LDH release during minutes 60 through 90. LVDP, Left ventricular developed pressure; LDH, lactate dehydrogenase. *P < .001 versus control or cardioplegia groups. The Journal of Thoracic and Cardiovascular Surgery 2005 130, 1151.e1-1151.e13DOI: (10.1016/j.jtcvs.2005.06.027) Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Myocardial abundance of p38 mitogen-activated protein kinase (p38, top) and protein-tyrosine kinase (JAK2, bottom) in time-matched control (open bar), ischemic (hatched bar), or cardioplegia-treated (filled bar) hearts after 2 hours of reperfusion or equivalent time for control hearts (n = 4). Units are relative to a common, nonischemic heart value set at 1.00. Western blotting shows representative hearts (n = 2) for each group. *P < .05 compared with control or cardioplegia-treated hearts. The Journal of Thoracic and Cardiovascular Surgery 2005 130, 1151.e1-1151.e13DOI: (10.1016/j.jtcvs.2005.06.027) Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Cardioplegia preserves expression of genes with cytoprotective function. Open bars represent unprotected ischemia, and filled bars represent cardioplegia-protected ischemia. Data are displayed relative to control values of 1, such that a 2-fold decrease (−2) is equivalent to 0.5. HSP70, 70-kd Heat shock protein; KATP-1, adenosine triphosphate–sensitive potassium channel uKATP-1; MAPK P-1, mitogen-activated protein kinase phosphatase 1; HSP70 precursor, 70-kd heat shock protein precursor; MAP3K8, serine-threonine protein kinase (Map3k8); PKC-e, protein kinase C ϵ; MnSOD, manganese superoxide dismutase; Heme Ox, heme oxygenase; HSP-related (hst70), heat shock protein-related gene hst70; SCS-3, suppressor of cytokine signaling 3; VDAC, voltage-dependent anion channel; p38 MAPK, p38 mitogen-activated protein kinase; JAK2, protein-tyrosine kinase. The Journal of Thoracic and Cardiovascular Surgery 2005 130, 1151.e1-1151.e13DOI: (10.1016/j.jtcvs.2005.06.027) Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions