Re-analysis of epidemiologically linked tuberculosis cases not supported by IS6110- RFLP-based genotyping A. Martín, J. Iñigo, F. Chaves, M. Herranz,

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Laboratory diagnosis and biosafety issues of biological warfare agents

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

P.-Y. Lévy Clinical Microbiology and Infection

Helicobacter pylori resistance to antibiotics in 2014 in France detected by phenotypic and genotypic methods A. Ducournau, L. Bénéjat, E. Sifré, E. Bessède,

Characteristics of Clostridium difficile strains isolated from asymptomatic individuals and from diarrheal patients S. Wongwanich, P. Pongpech, C. Dhiraputra,

L. Boyanova Clinical Microbiology and Infection

Gut bacterial microbiota and obesity

Approach to diagnosis of infective endocarditis

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated over a 2-year period in a Qatari hospital from multinational patients

Prolonged viral shedding in pandemic influenza A(H1N1): clinical significance and viral load analysis in hospitalized patients M. Giannella, M. Alonso,

Herpes zoster in non-hospitalized children

High-level quinolone resistance is associated with the overexpression of smeVWX in Stenotrophomonas maltophilia clinical isolates G. García-León, C.

Mycobacterial peritonitis: difference between non-tuberculous mycobacteria and Mycobacterium tuberculosis C.-C. Shu, J.-T. Wang, J.-Y. Wang, C.-J. Yu,

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

J.-P. Van geertruyden Clinical Microbiology and Infection

A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial.

Fast and low-cost decentralized surveillance of transmission of tuberculosis based on strain-specific PCRs tailored from whole genome sequencing data:

Virological tools to diagnose and monitor hepatitis C virus infection

M. Montes, D. Vicente, O. Esnal, G. Cilla, E. Pérez-Trallero

Assessment of fluorescent amplified fragment length polymorphism analysis for epidemiological genotyping of Legionella pneumophila serogroup 1 N.K. Fry,

The use of AP-PCR and flaA-RFLP typing to investigate treatment failure in Helicobacter pylori infection I. Adamsson, C. Edlund, R. Seensalu, L. Engstrand

R. Cantón Clinical Microbiology and Infection

Genital Chlamydia trachomatis infections

How to evaluate and predict the ecologic impact of antibiotics: the pharmaceutical industry view from research and development R. Bax Clinical Microbiology.

Impact of immigration on tuberculosis in a low-incidence area of Italy: a molecular epidemiological approach C. Garzelli, N. Lari, B. Cuccu, E. Tortoli,

How to evaluate and predict the epidemiologic impact of antibiotic use in humans: the pharmacoepidemiologic approach D. Guillemot Clinical Microbiology.

W. Sougakoff Clinical Microbiology and Infection

Holt Hanne M. , Søgaard Per , Gahrn-Hansen Bente

Impact of antibiotic restrictions: the pharmaceutical perspective

Vector control: a cornerstone in the malaria elimination campaign

Differences in the robustness of clusters involving the Mycobacterium tuberculosis strains most frequently isolated from immigrant cases in Madrid N.

S. M. Arend, E. Cerdé de Palou, P. de Haas, R. Janssen, M. A. Hoeve, E

Assessment of fluorescent amplified fragment length polymorphism analysis for epidemiological genotyping of Legionella pneumophila serogroup 1 N.K. Fry,

Molecular epidemiology of Staphylococcus aureus strains colonizing the lungs of related and unrelated cystic fibrosis patients Nicole H.M. Renders, Alex.

Training for the infectious diseases speciality in Norway

Differential findings regarding molecular epidemiology of tuberculosis between two consecutive periods in the context of steady increase of immigration

F. Grill, P. Muñoz, R. Jofre, E. Bouza

Investigation of suspected laboratory cross-contamination: interpretation of single smear-negative, positive cultures for Mycobacterium tuberculosis

A novel method for the rapid and prospective identification of Beijing Mycobacterium tuberculosis strains by high-resolution melting analysis M. Alonso,

Laboratory diagnosis and biosafety issues of biological warfare agents

Lack of association between genotypes and haematogenous seeding infections in a large cohort of patients with methicillin-resistant Staphylococcus aureus.

S. Godreuil, F. Renaud, M. Choisy, J. J. Depina, E. Garnotel, M

Microbiological and epidemiological studies of Enterococcus faecium resistant to amoxycillin in a university hospital in eastern France M. Thouverez,

S.-M. Sheu, B.-S. Sheu, C.-C. Lu, H.-B. Yang, J.-J. Wu

Metagenomics and probiotics

Transmission permeability of tuberculosis involving immigrants, revealed by a multicentre analysis of clusters N. Alonso Rodríguez, S. Andrés, E. Bouza,

Previously unknown species of Aspergillus

Laboratory diagnosis of Clostridium difficile disease

New developments in laboratory monitoring of HIV-1 infection

Contact investigations for outbreaks of Mycobacterium tuberculosis: advances through whole genome sequencing T.M. Walker, P. Monk, E. Grace Smith, T.E.A.

B.J.A. Rijnders, E. van Wijngaerden, J. van Eldere, W.E. Peetermans

Molecular epidemiology and virulence factors of pyogenic liver abscess causing Klebsiella pneumoniae in China Y. Luo, Y. Wang, L. Ye, J. Yang Clinical.

Training in infectious diseases and tropical medicine in Britain

Tuberculosis transmission patterns among Spanish-born and foreign-born populations in the city of Barcelona S. Borrell, G. Tudó, E. Rey, J. González-Martín

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

Clinical infection services—the Leiden experience

The use of AP-PCR and flaA-RFLP typing to investigate treatment failure in Helicobacter pylori infection I. Adamsson, C. Edlund, R. Seensalu, L. Engstrand

L. R. Ásmundsdóttir, H. Erlendsdóttir, A. L. Gísladóttir, M

Statin use and clinical outcomes among pneumonia patients

Amplified fragment length polymorphism genotyping of metronidazole-resistant Helicobacter pylori infecting dyspeptics in England R.J. Owen, M. Ferrus,

F.J. Pastor, J. Guarro Clinical Microbiology and Infection

J.J. Keller, M.-C. Tsai, C.-C. Lin, Y.-C. Lin, H.-C. Lin

Facilitating learning and change in physicians: Implications for a system of continuing medical education in Europe Robert D. Fox Clinical Microbiology.

Abstracts Clinical Microbiology and Infection

Javier Aznar, Hassan Safi, Maria C. Conejo, José C. Palomares

G.C. Schito Clinical Microbiology and Infection

F. Franzetti, L. Codecasa, A. Matteelli, A. Degli Esposti, A

Impact of antibiotic restrictions: the patient's perspective

The future of diagnostic bacteriology

Presentation transcript:

Re-analysis of epidemiologically linked tuberculosis cases not supported by IS6110- RFLP-based genotyping A. Martín, J. Iñigo, F. Chaves, M. Herranz, M.J. Ruiz-Serrano, E. Palenque, E. Bouza, D. García de Viedma Clinical Microbiology and Infection Volume 15, Issue 8, Pages 763-769 (August 2009) DOI: 10.1111/j.1469-0691.2009.02839.x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1. Microepidemics showing cases matched using molecular techniques. N, number of cases involved in each microepidemic; RFLP, restriction fragment length polymorphism. Clinical Microbiology and Infection 2009 15, 763-769DOI: (10.1111/j.1469-0691.2009.02839.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2. Linked pairs initially considered not supported by the molecular analysis. The figure shows the number identifying each linked pair, the patients involved, the number of isolates available (I.A.) for analysis from independent specimens, the restriction fragment length polymorphism (RFLP), spoligotype, and the Mycobacterial interspersed repetitive unit (MIRU) patterns. MIRU alleles with differences between the cases involved are highlighted in bold. For case 5112-2, who was infected with two clonal variants, both alleles are shown. Pairs are grouped as follows according to the redistribution in different interpretation categories after the refined molecular re-analysis: (a) proved linked pairs after identifying a laboratory error, (b) proved linked pairs for which a molecular match had been established after second-line genotyping, (c) pairs with partial molecular differences in second-line genotyping, and (d) pairs with marked molecular differences in second-line genotyping. Clinical Microbiology and Infection 2009 15, 763-769DOI: (10.1111/j.1469-0691.2009.02839.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3. Summary of the re-analysis of the 44 pairs initially considered discrepant using molecular data. From the total number of pairs in each panel, the number of pairs that involved household contacts is also indicated. Clinical Microbiology and Infection 2009 15, 763-769DOI: (10.1111/j.1469-0691.2009.02839.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions