RBR Ubiquitin Transfer: Not Simply an “Open” and “Closed” Case? Karen M. Dunkerley, Gary S. Shaw  Structure  Volume 25, Issue 6, Pages 817-819 (June 2017) DOI: 10.1016/j.str.2017.05.013 Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 1 Structure of the RBR E3 Ligase HHARI Bound to the UbcH7∼Ub Conjugate (A) A cartoon structure of the HHARI/UbcH7∼Ub complex (Dove et al., 2017) showing the association of UbcH7 with the RING1 domain and “open” conformation of the conjugated Ub (∼Ub). (B) As in (A) but with ∼Ub in the analogous position observed in the NEDD4L/UbcH5b∼Ub complex (PDB: 3UGB). The arrow indicates an additional C-terminal helix in the UBA-L domain that is not observed in apo-HHARI structures. (C) As in (A) with the ∼Ub replaced by that observed in the HOIP/UbcH5b∼Ub complex (PDB: 5EDV). The arrow indicates the observed Ub binding region at the N terminus of the RING2/Rcat domain (Dove et al., 2016). Structure 2017 25, 817-819DOI: (10.1016/j.str.2017.05.013) Copyright © 2017 Elsevier Ltd Terms and Conditions