Detection of enteroviral infection in myocardial tissues by polymerase chain reaction (PCR)  Mario Marianil, Anna S. Petronio, Maria T. Manes, Marcello Morelli, Donatella Squecco, Hncenxo Nardini, Generoso Bevilacqua, Luca Ceccherini-Nelli  Clinical Microbiology and Infection  Volume 2, Issue 2, Pages 109-114 (October 1996) DOI: 10.1111/j.1469-0691.1996.tb00215.x Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Southern blot analysis of cDNA PCR products of acutely infected mouse myocardial tissue. K+. 0.1 μg total RNA from KB infected cells with CVB3: K−1, 0.1 μg total RNA from KB uninfected cells: K−2, uninfected mouse myocardial tissue: A. analyzed mouse myocardial tissue at 12 h from infection; C, analyzed mouse myocardial tissue at 24 h from infection; C, analyzed mouse myocardial tissue at 36 h from infection: D analyzed mouse myocardial tissue at 48 h from infection. Seven microliters of EDTA solution containing RNA from these tissues were used for cDNA PCR analysis as previously described. All amplified products were hybridized with a CVB3 548-568 terminally 32P-labeled probe at 42°C overnight, and washed at 55°C in 5xSSC, 0.1% SDS. Clinical Microbiology and Infection 1996 2, 109-114DOI: (10.1111/j.1469-0691.1996.tb00215.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Southern blot analysis of endomyocardial biopsies amplified by cDNA PCR. 1, Histological picture of borderline myocarditis (patient no. 6); 2, histologic picture of active myocarditis (patient no. 2); 3, histologic picture of non-specific damage (patient no. 26); 4, histologic picture of dilated cardiomyopathy (patient no. 11, endomyocardial biopsied tissue); 5, histologic picture of dilated cardiomyopathy (patient no. 10, endomyocardial autopsy tissue); X, H2O (no nucleic acid); K–, 0.1 μg KB uninfected cell RNA; K+, 0.1 μg KB CVB3 infected cell RNA. Seven microliters of EDTA solution containing RNA from endomyocardial biopsied and expanted tissues were used for cDNA PCR analysis. Labeled CVB3 probe and hybridization procedure are the same used for mouse myocardial tissue. Clinical Microbiology and Infection 1996 2, 109-114DOI: (10.1111/j.1469-0691.1996.tb00215.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions