Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes Emma M. Savilahti, MD, Ville.

Slides:

Advertisements

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Advertisements

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Probiotics prevent IgE-associated allergy until age 5 years in cesarean-delivered children but not in the total cohort Mikael Kuitunen, MD, PhD, Kaarina.

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns Christian S. Hansen, PhD, Martin Dufva,

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

The Natural History of Food Allergy

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity Fabio Dall'Antonia, PhD, Anna Gieras,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy Julie Wang, MD,

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns Christian S. Hansen, PhD, Martin Dufva,

Is 9 more than 2 also in allergic airway inflammation?

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Allergy testing in predicting outcome of open food challenge to peanut

Jing Lin, PhD, Ludmilla Bardina, MSc, Wayne G

Immune factors in breast milk related to infant milk allergy are independent of maternal atopy Kirsi M. Järvinen, MD, PhD, Mayte Suárez-Fariñas, PhD,

Physician needs in health informatics: Just ask the docs

Predicting development of sustained unresponsiveness to milk oral immunotherapy using epitope-specific antibody binding profiles Mayte Suárez-Fariñas,

Elina Jerschow, MD, MSc, Robert Y. Lin, MD, MSc, Moira M

Peter M. Wolfgram, MD, David B. Allen, MD

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Greater epitope recognition of shrimp allergens by children than by adults suggests that shrimp sensitization decreases with age Rosalía Ayuso, MD, PhD,

An age-dependent association of mannose-binding lectin-2 genetic variants on HIV-1– related disease in children Kumud K. Singh, PhD, Alexis Lieser, MD,

Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy Annebeth E. Flinterman, MD, Edward F. Knol,

Debra J. Palmer, PhD, Thomas R. Sullivan, BMa&CompSc(Hons), Michael S

Staphylococcus aureus colonization is associated with wheeze and asthma among US children and young adults Meghan F. Davis, PhD, Roger D. Peng, PhD,

Is eczema really on the increase worldwide?

Anna Nowak-Wegrzyn, MD, Katherine A. Bloom, MD, Scott. H

Peanut oral immunotherapy modifies IgE and IgG4 responses to major peanut allergens Brian P. Vickery, MD, Jing Lin, PhD, Michael Kulis, PhD, Zhiyan Fu,

An experimental and modeling-based approach to locate IgE epitopes of plant profilin allergens Gema López-Torrejón, PhD, Araceli Díaz-Perales, PhD, Julia.

Identification of IgE- and IgG-binding epitopes on αs1-casein: Differences in patients with persistent and transient cow's milk allergy Pantipa Chatchatee,

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

Claus Bachert, MD, PhD, Cezmi A. Akdis, MD

Weiguo Chen, PhD, Gurjit K. Khurana Hershey, MD, PhD

Medication use in children with asthma in Finland from 1995 to 2006

The widening black/white gap in asthma hospitalizations and mortality

Audrey DunnGalvin, PhD, L. M

Determination of IgG subclasses: A need for standardization

Autophagy: Nobel Prize 2016 and allergy and asthma research

Influence of early-life exposures on food sensitization and food allergy in an inner-city birth cohort Emily C. McGowan, MD, Gordon R. Bloomberg, MD,

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Diagnosis and management of early asthma in preschool-aged children

A single intervention for cockroach control reduces cockroach exposure and asthma morbidity in children Felicia A. Rabito, PhD, MPH, John C. Carlson,

B-cell epitopes as a screening instrument for persistent cow's milk allergy Kirsi-Marjut Järvinen, MD, PhD, Kirsten Beyer, MD, Leticia Vila, MD, Pantipa.

Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R

Time trends in Australian hospital anaphylaxis admissions in to

The natural history of IgE-mediated cow's milk allergy

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Exposure to cow’s milk during the first 3 months of life is associated with increased levels of IgG subclass antibodies to β-lactoglobulin to 8 years

The natural history of egg allergy in an observational cohort

Measles and immunomodulation

Macrolide antibiotics and asthma treatment

Asthma: The past, future, environment, and costs

Identification of IgE sequential epitopes of lentil (Len c 1) by means of peptide microarray immunoassay Andrea Vereda, MD, PhD, Doerthe A. Andreae,

The natural history of milk allergy in an observational cohort

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

Personal and parental nativity as risk factors for food sensitization

Food allergy is associated with Staphylococcus aureus colonization in children with atopic dermatitis Andrea L. Jones, MD, Douglas Curran-Everett, PhD,

Environmental factors and eosinophilic esophagitis

The prevalence and natural course of food protein–induced enterocolitis syndrome to cow’s milk: A large-scale, prospective population-based study Yitzhak.

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

Advances in pediatric asthma in 2007

Allergen-specific IgG antibody levels modify the relationship between allergen-specific IgE and wheezing in childhood Adnan Custovic, MD, PhD, Lars Soderstrom,

Monitoring the epitope recognition profiles of IgE, IgG1, and IgG4 during birch pollen immunotherapy Barbara Gepp, MSc, Nina Lengger, BMA, Christian.

Natural history of cow’s milk allergy

The Editors’ Choice Journal of Allergy and Clinical Immunology

News Beyond Our Pages Journal of Allergy and Clinical Immunology

Presentation transcript:

Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes Emma M. Savilahti, MD, Ville Rantanen, MSc, Jing S. Lin, PhD, Sirkku Karinen, MSc, Kristiina M. Saarinen, MD, PhD, Marina Goldis, MD, Mika J. Mäkelä, MD, PhD, Sampsa Hautaniemi, DTech, Erkki Savilahti, MD, PhD, Hugh A. Sampson, MD Journal of Allergy and Clinical Immunology Volume 125, Issue 6, Pages 1315-1321.e9 (June 2010) DOI: 10.1016/j.jaci.2010.03.025 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Differences in IgE binding to CM peptides (black curve) between children with persisting CMA at the age of 8 to 9 years (P, red) and those recovering early by the age of 3 years (E, green) at 3 time points: A, T:0, the time of diagnosis; B, T:1, 1 year later; C, T:9, follow-up at the age of 8 to 9 years. Arrows point to regions with significant (>0.1 or <−0.1) differences between groups. The x-axis depicts amino acid sequences of the 5 proteins, and the y-axis depicts relative signal strength. The temporal differences in IgE-binding intensity are shown within study groups (D) and between groups (E); red denotes regions with significantly (>0.1) increased binding intensity, and blue denotes regions with significantly (<−0.1) decreased binding intensity. β-LG, β-Lactoglobulin. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Differences in IgG4 binding to CM peptides (black curve) between children with persisting CMA at the age of 8 to 9 years (P, red) and those recovering early by the age of 3 years (E, green) at 3 time points: A, T:0, the time of diagnosis; B, T:1, 1 year later; C, T:9, follow-up at the age of 8 to 9 years. Arrows point to regions with significant (>0.1 or <−0.1) differences between groups. The x-axis depicts amino acid sequences of the 5 proteins, and the y-axis depicts relative signal strength. The temporal differences in IgG4-binding intensity are shown within study groups (D) and between groups (E); red denotes regions with significantly (>0.1) increased binding intensity, and blue denotes regions with significantly (<−0.1) decreased binding intensity. β-LG, β-Lactoglobulin. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 A, Differences in IgA binding to CM peptides (black curve) between children with persisting CMA at the age of 8 to 9 years (P, red) and those recovering early by the age of 3 years (E, green) at the time of follow-up at the age of 8 to 9 years (T:9). Arrows point to regions with significant (>0.1 or <−0.1) differences between groups. The x-axis depicts amino acid sequences of the 5 proteins, and the y-axis depicts relative signal strength. The temporal differences in IgA-binding intensity are shown within study groups (B) and between groups (C); red denotes regions with significantly (>0.1) increased binding intensity, and blue denotes regions with significantly (<−0.1) decreased binding intensity. β-LG, β-Lactoglobulin. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Differences in overlap of antibody-binding intensity to CM peptides between children with persisting CMA at the age of 8 to 9 years (P) and those recovering early by the age of 3 years (E). Time points are denoted as follows: T:0, the time of diagnosis; T:1, 1 year later; T:9, follow-up at the age of 8 to 9 years. Red denotes regions where the difference of binding intensity between IgE and IgG4 (A) or between IgE and IgA (B) is greater (less overlap) in group P compared with that in group E, and blue denotes the opposite. Differences of greater than 0.1 or less than −0.1 are considered significant. β-LG, β-Lactoglobulin. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

A theoretical situation in which only a single peptide is active A theoretical situation in which only a single peptide is active. The Gaussian convolution kernel is shown as a dashed line. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

The signal of one single active peptide after convolution with a Gaussian function. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

A theoretical situation where four peptides are active A theoretical situation where four peptides are active. The Gaussian convolution kernel is shown as a dashed line. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

The signal of four active peptides after convolution with a Gaussian function. Note that the peptides closer to each other form a higher signal. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

The individual sample signal strengths after convolution (thin blue line) and their averages (thick black line). Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Differences (black curve) between IgE (orange) and IgG4 (blue) binding to CM peptides in children recovering early by the age of 3 years from CMA at 3 time points: A, T:0, the time of diagnosis; B, T:1, 1 year later; C, T:9, follow-up at the age of 8 to 9 years. Arrows point to regions with significant (>0.1 or <−0.1) differences between IgE and IgG4 binding. The x-axis depicts amino acid sequences of the 5 proteins, and the y-axis depicts relative signal strength. β-LG, β-Lactoglobulin. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Differences (black curve) between IgE (orange) and IgG4 (blue) binding to CM peptides in children with persisting CMA at the age of 8 to 9 years at 3 time points: A, T:0, the time of diagnosis; B, T:1, 1 year later; C, T:9, follow-up at the age of 8 to 9 years. Arrows point to regions with significant (>0.1 or <−0.1) differences between IgE and IgG4 binding. The x-axis depicts amino acid sequences of the 5 proteins, and the y-axis depicts relative signal strength. β-LG, β-Lactoglobulin. Journal of Allergy and Clinical Immunology 2010 125, 1315-1321.e9DOI: (10.1016/j.jaci.2010.03.025) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions