Monitoring inflammatory heterogeneity with multiple biomarkers for multidimensional endotyping of asthma Ioana Agache, MD, PhD, Daniel S. Strasser, PhD,

Slides:

Advertisements

Similar presentations

Quartiles & Extremes (displayed in a Box-and-Whisker Plot) Lower Extreme Lower Quartile Median Upper Quartile Upper Extreme Back.

Advertisements

Making a Box & Whiskers Plot Give Me Five!. 5 Numbers are Needed 1) Lowest: Least number of the data set 2) Lower Quartile : The median of the lower half.

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Date of download: 10/12/2017 Copyright © ASME. All rights reserved.

a graphical presentation of the five-number summary of data

D-dimer: A biomarker for antihistamine-resistant chronic urticaria

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease Merja T. Augustin, MD, Jorma Kokkonen, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease Merja T. Augustin, MD, Jorma Kokkonen, MD,

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.

Allergen immunotherapy: A practice parameter third update

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD

Chlamydophila (Chlamydia) pneumoniae serology and asthma in adults: A longitudinal analysis Rafael Pasternack, MD, PhD, Heini Huhtala, MSc, Jussi Karjalainen,

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Jan Lötvall, MD, Cezmi A. Akdis, MD, Leonard B

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD

The Editors' Choice Journal of Allergy and Clinical Immunology

BOX-and-WHISKER PLOT (Box Plot)

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD

Is 9 more than 2 also in allergic airway inflammation?

Desensitization to Chemotherapeutic Agents

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

John W. Steinke, PhD, Spencer C. Payne, MD, M

Clara cell 16-kd protein downregulates TH2 differentiation of human naive neonatal T cells Sofi Johansson, MSc, Göran Wennergren, MD, PhD, Nils Åberg,

Silvana Balzar, MD, Matthew Strand, PhD, Diane Rhodes, PhD, Sally E

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Timothy S. C. Hinks, MD, Xiaoying Zhou, PhD, Karl J

Peter A. B. Wark, BMed, PhD, Fabio Bucchieri, MD, Sebastian L

Multidimensional endotyping in patients with severe asthma reveals inflammatory heterogeneity in matrix metalloproteinases and chitinase 3–like protein.

Melissa M. Garrido, PhD, Holly G

The future of allergy/immunology: Promising young investigators

Ordinary vibratory angioedema is not generally associated with ADGRE2 mutation Zuotao Zhao, MD, PhD, Sascha Reimann, MD, Shan Wang, MD, Yuhan Wang, MD,

Roles of arginase variants, atopy, and ozone in childhood asthma

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Food allergy in patients with primary immunodeficiency diseases: Prevalence within the US Immunodeficiency Network (USIDNET) Karen S. Tuano, MD, Jordan.

Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy Katharina Dreschler, Kai Bratke, PhD, Sebastian Petermann,

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

Christian Hennig, MD, Christine Happle, MD, Gesine Hansen, MD

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS

Claus Bachert, MD, PhD, Cezmi A. Akdis, MD

Altered metabolic profile in patients with IgE to galactose-alpha-1,3-galactose following in vivo food challenge John W. Steinke, PhD, Shawna L. Pochan,

Serum ferritin and transferrin levels are not serologic markers of toluene diisocyanate– induced occupational asthma Joaquín Sastre, MD, PhD, Beatriz.

What is an “eosinophilic phenotype” of asthma?

Medication use in children with asthma in Finland from 1995 to 2006

Topical antifungal treatment of chronic rhinosinusitis with nasal polyps: A randomized, double-blind clinical trial Michael Weschta, MD, Dagmar Rimek,

BOX-and-WHISKER PLOT (Box Plot)

Personalized asthma therapy in blacks—the role of genetic ancestry

Cockroach allergens: Coping with challenging complexity

Autophagy: Nobel Prize 2016 and allergy and asthma research

Advances in the approach to the patient with food allergy

One step forward, 2 steps back: The enigma of preschool wheeze

Benjamin Chaigne, MD, Hervé Watier, MD, PhD

Martin Brasholt, MD, Florent Baty, PhD, Hans Bisgaard, MD, DMSci

Contribution of dust mite and cat specific IgE to total IgE: Relevance to asthma prevalence Elizabeth A. Erwin, MD, Eva Rönmark, PhD, Kristin Wickens,

Reply Journal of Allergy and Clinical Immunology

Macrolide antibiotics and asthma treatment

Asthma: The past, future, environment, and costs

Interrupting IL-6–receptor signaling improves atopic dermatitis but associates with bacterial superinfection Alexander A. Navarini, MD, PhD, Lars E.

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

Environmental factors and eosinophilic esophagitis

News & Notes Journal of Allergy and Clinical Immunology

Double-blind, placebo-controlled study with a modified therapeutic vaccine of Salsola kali (Russian thistle) administered through use of a cluster schedule

Safety and efficacy of repeated monthly carboplatin desensitization

Effect of codeine on objective measurement of cough in chronic obstructive pulmonary disease Jaclyn Smith, MD, PhD, Emily Owen, MPhil, John Earis, MD,

Endogenous bradykinin and B1-B5 during angiotensin-converting enzyme inhibitor– associated angioedema Scott A. Hubers, MD, Kevin Kohm, MS, Shouzuo Wei,

Natural history of cow’s milk allergy

Primary prevention of asthma and allergy

Presentation transcript:

Monitoring inflammatory heterogeneity with multiple biomarkers for multidimensional endotyping of asthma Ioana Agache, MD, PhD, Daniel S. Strasser, PhD, Gabin M. Pierlot, Hervé Farine, PhD, Kenji Izuhara, MD, PhD, Cezmi A. Akdis, MD, PhD Journal of Allergy and Clinical Immunology Volume 141, Issue 1, Pages 442-445 (January 2018) DOI: 10.1016/j.jaci.2017.08.027 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 TDA was used to analyze and visualize clustering of subjects based on their similarity across 21 clinical, physiologic, and inflammatory parameters simultaneously. TDA constructs a network of nodes with edges between them. The nodes represent sets of data points, and 2 nodes are connected with an edge if their corresponding collections of data points have a point in common. A patient can fall into several nodes, which is how 2 nodes are connected. More precisely, we connected 2 nodes with an edge if they shared at least 1 patient. The filter function was set to principal and secondary singular value decomposition. The number of intervals was 10 and 10, respectively, and the overlapping percentage was fixed to 50. The original TDA network is simplified by focusing on nodes with more than 4 patients. Clusters are visualized by 2 using dimensions: asthma severity (GINA) and blood eosinophilia. Nodes are colored based on severity, and their size is proportional to the number of patients, which is also indicated numerically inside the node. Connecting nodes were assigned to a particular cluster based on either patient overrepresentation in the neighboring nodes or the number of connections within a cluster. Six clusters were identified. Eight patients did not cluster and appeared to represent a less common individual endotype. Journal of Allergy and Clinical Immunology 2018 141, 442-445DOI: (10.1016/j.jaci.2017.08.027) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 The 12 most relevant clinical characteristics and serum biomarkers are visualized as box plots for the 6 TDA clusters. Dots represent outliers, and the box represents the second, median, and third quartiles (50% of the data points). The upper whisker goes to the highest value that is within 1.5*interquartile range (IQR), and the lower whisker goes to the lowest value that is within 1.5*IQR (opposite direction). The rest of data points are called outliers and represented as dots. ACT, Asthma Control Test; MCP-1, monocyte chemoattractant protein 1. Journal of Allergy and Clinical Immunology 2018 141, 442-445DOI: (10.1016/j.jaci.2017.08.027) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Journal of Allergy and Clinical Immunology 2018 141, 442-445DOI: (10.1016/j.jaci.2017.08.027) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Journal of Allergy and Clinical Immunology 2018 141, 442-445DOI: (10.1016/j.jaci.2017.08.027) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Journal of Allergy and Clinical Immunology 2018 141, 442-445DOI: (10.1016/j.jaci.2017.08.027) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Journal of Allergy and Clinical Immunology 2018 141, 442-445DOI: (10.1016/j.jaci.2017.08.027) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions