Phosphatase activity associated with Themis and Shp1 in response to different affinity peptide ligands. Phosphatase activity associated with Themis and.

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Phosphatase activity associated with Themis and Shp1 in response to different affinity peptide ligands. Phosphatase activity associated with Themis and Shp1 in response to different affinity peptide ligands. Tyrosine phosphatase activity assay with (A) immunoprecipitated Themis from OT-I CTLs in response to different affinity peptide ligands using Themis−/− thymocytes as a negative control. (B) Immunoprecipitated Shp1 from OT-I CTLs in response to the use of different affinity peptide ligands along with unstimulated CTLs. (C) Immunoprecipitated Shp1 from CTLs made from OT-I Themis+/+ and OT-I Themis−/−, comparing unstimulated and OVA-stimulated cells. (D) Immunoprecipitated Shp1, showing reduction in Shp1 activity in OT-I β2m−/−Themis−/− thymocytes compared with OT-I β2m−/−Themis+/+ thymocytes in response to different stimulations along with unstimulated control. (E) Immunoprecipitated Themis in OT-I β2m−/−Themis+/+ thymocytes in response to stimulations using different affinity peptide ligands and OT-I β2m−/−Themis−/− thymocytes as a negative control. All of the stimulations were done for 90 s. Data are representative of three independent experiments. Unpaired t test with mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Monika Mehta et al. PNAS 2018;115:48:E11331-E11340 ©2018 by National Academy of Sciences