A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial.

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Presentation transcript:

A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence L. Pérez-Lago, S. Izco, M. Herranz, G. Tudó, M. Carcelén, I. Comas, O. Sierra, J. González-Martín, M.J. Ruiz-Serrano, J. Eyene, E. Bouza, D. García de Viedma Clinical Microbiology and Infection Volume 23, Issue 2, Pages 92-97 (February 2017) DOI: 10.1016/j.cmi.2016.10.006 Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 (a) Amplification patterns obtained from EG-MDR strain-specific multiplex ASO-PCR results for control EG-MDR and non-EG-MDR strains. Amplicons corresponding to each of four targeted SNPs are indicated. (b) Selection of EG-MDR and non-EG-MDR strains. ASO, allele-specific oligonucleotide; EG-MDR, multidrug-resistant prevalent tuberculosis strain in Equatorial Guinea; MW, 100 bp ladder; SNP, single nucleotide polymorphism. Clinical Microbiology and Infection 2017 23, 92-97DOI: (10.1016/j.cmi.2016.10.006) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Results obtained after applying EG-MDR strain-specific multiplex ASO-PCR to selection of historical isolates. Isolates identified as EG-MDR or non-EG-MDR are indicated. ASO, allele-specific oligonucleotide; EG-MDR, multidrug-resistant prevalent tuberculosis strain in Equatorial Guinea; MW, 50 bp ladder. Clinical Microbiology and Infection 2017 23, 92-97DOI: (10.1016/j.cmi.2016.10.006) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions