Multifocal superresolved (MFM STORM) image of mitochondria.

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Multifocal superresolved (MFM STORM) image of mitochondria. Multifocal superresolved (MFM STORM) image of mitochondria. TOM20–GFP-expressing HeLa cells were labeled with anti-GFP nanobody Alexa 647 conjugate. (A) Image recorded by wide-field MFM. (Scale bar: 5 μm.) (B) Image recorded by MFM STORM. Single Alexa 647 fluorophores are detectable at different focal planes. (Scale bar: 5 μm.) (C) Reconstructed 3D superresolution image (the depth is color-coded) (Movies S3–S5). The localizations are represented as isotropic 3D Gaussian profiles of 20-nm SD using the 3D superresolution reconstruction software ViSP (29). (Scale bar: 2 μm.) (D and E) Enlarged view of area 1 in C showing the lateral and axial views, respectively. (Scale bar: 500 nm.) (F and G) Detection density profile of structure 2 in C plotted laterally and axially, respectively. (H) Cross-section of the structure shown in F and G showing mitochondrial lumen (arrow = 100 nm). Bassam Hajj et al. PNAS 2014;111:49:17480-17485 ©2014 by National Academy of Sciences