Pol I promoters. Pol III promoters.

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Presentation transcript:

Pol I promoters.

Pol III promoters.

Pol II promoters.

The pol II CTD: new twists in the tail The pol II CTD: new twists in the tail. Justyna Zaborowska, Sylvain Egloff & Shona MurphyNature Structural & Molecular Biology 23, 771–777 (2016)

The pol II CTD: new twists in the tail The pol II CTD: new twists in the tail. Justyna Zaborowska, Sylvain Egloff & Shona MurphyNature Structural & Molecular Biology 23, 771–777 (2016)

The pol II CTD: new twists in the tail The pol II CTD: new twists in the tail. Justyna Zaborowska, Sylvain Egloff & Shona MurphyNature Structural & Molecular Biology 23, 771–777 (2016)

Fig. 1. Transcription PIC. Class II gene transcription is brought about by (in humans) over a hundred polypeptides assembling on the core promoter of protein-encoding genes, which then give rise to messenger RNA. A PIC on a core promoter is shown in a schemati... Kapil Gupta, Duygu Sari-Ak, Matthias Haffke, Simon Trowitzsch, Imre Berger Zooming in on Transcription Preinitiation Journal of Molecular Biology, Volume 428, Issue 12, 2016, 2581–2591 http://dx.doi.org/10.1016/j.jmb.2016.04.003

Figure 2. Structure of the promoter-bound core of TFIID Figure 2. Structure of the promoter-bound core of TFIID. The cryo-EM reconstruction of the promoter-bound core of TFIID comprising lobes A1, B and C, is shown here colored according to current subunit assignments and with available atomic models docked (EMD-330520). The promoter DNA and the locations of the sequence motifs present in the super core promoter used in the experiment are also modeled. Note that the positions and identities of the TAFs making up lobe B (light blue) are yet unknown, owing to the flexibility and thus lower resolution of this region. For clarity, the flexible part of lobe A (lobe A2) is not shown (see Fig. 1). Published in: Eva Nogales; Jie Fang; Robert K. Louder; Transcription 2017, 8, 55-60. DOI: 10.1080/21541264.2016.1265701 Copyright © 2017 The Author(s)

Figure 3. Model of the TFIID-based PIC Figure 3. Model of the TFIID-based PIC. Superposition of the common elements in the TBP-based PIC (upper left) and the TFIID–IIA–DNA complex (lower left). Superposition of TBP, TFIIA and promoter DNA (shown in ribbon representation) result in a synthetic structural model for the TFIID-based PIC (right,20). The coloring scheme for the PIC components is shown on the bottom, with the TFIID subunits colored similarly as in Fig. 2. Published in: Eva Nogales; Jie Fang; Robert K. Louder; Transcription 2017, 8, 55-60. DOI: 10.1080/21541264.2016.1265701 Copyright © 2017 The Author(s)