Methylation status of human chorionic gonadotropin beta subunit promoter and TFAP2A expression as factors regulating CGB gene expression in placenta 

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Methylation status of human chorionic gonadotropin beta subunit promoter and TFAP2A expression as factors regulating CGB gene expression in placenta  Aleksandra Głodek, Ph.D., Marta J. Kubiczak, M.Sc., Grzegorz P. Walkowiak, M.Sc., Ewa Nowak-Markwitz, Anna Jankowska, Ph.D.  Fertility and Sterility  Volume 102, Issue 4, Pages 1175-1182.e8 (October 2014) DOI: 10.1016/j.fertnstert.2014.06.016 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Relative expression level of (A) CGB1-9, (B) CGB3–9, and (C) CGB1–2 in chorionic (CT) and placental (PT) tissue. Fertility and Sterility 2014 102, 1175-1182.e8DOI: (10.1016/j.fertnstert.2014.06.016) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Relative expression level of (A) SP1, (B) SP3, (C) SP1/SP3, and (D) TFAP2A in chorionic (CT) and placental (PT) tissue. Fertility and Sterility 2014 102, 1175-1182.e8DOI: (10.1016/j.fertnstert.2014.06.016) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Methylation status of (A, B) CGB3–9 and (C, D) CGB1–2 promoter sequence in chorionic (CT) and placental (PT) tissue. Fertility and Sterility 2014 102, 1175-1182.e8DOI: (10.1016/j.fertnstert.2014.06.016) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 CGB genes promoter sequence alignment performed according to the ClustalW2 algorithm. Parallel arrows indicate AP2 and Sp1 binding sites, while the kinked arrow shows transcription start site; frames mark CpGs analyzed in MSP assays. Fertility and Sterility 2014 102, 1175-1182.e8DOI: (10.1016/j.fertnstert.2014.06.016) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions