Donor Heart Preservation in an Empty Beating State Under Mild Hypothermia Hui Lin, MD, Ansheng Mo, MD, Fan Zhang, MD, Ailan Huang, MM, Zhaoke Wen, MM,

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Donor Heart Preservation in an Empty Beating State Under Mild Hypothermia Hui Lin, MD, Ansheng Mo, MD, Fan Zhang, MD, Ailan Huang, MM, Zhaoke Wen, MM, Shengjin Ling, MM, Yanyan Hu, MM, Yifang Zhou, MM, Changchao Lu, MM The Annals of Thoracic Surgery Volume 89, Issue 5, Pages 1518-1523 (May 2010) DOI: 10.1016/j.athoracsur.2010.02.008 Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Schematic of perfusion device and the cardiopulmonary bypass for the heart. The perfusion device consisted of a roller pump, small heat exchanger, gas blender, oxygenator of infant and blood reservoir, self-design heart preservation chamber, filtration system, and tubing system. Blood circulation flowed from ascending aortic cannula to coronary ostia to coronary sinus to blood reservoir to pump to membrane oxygenator to filter, and back to ascending aortic cannula. The Annals of Thoracic Surgery 2010 89, 1518-1523DOI: (10.1016/j.athoracsur.2010.02.008) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Perfusion the heart and decompression of the heart chambers. A cannula for perfusion was inserted through the brachiocephalic artery, and a catheter for decompression was introduced into the left ventricle through the pulmonary vein and mitral valve. The Annals of Thoracic Surgery 2010 89, 1518-1523DOI: (10.1016/j.athoracsur.2010.02.008) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Electron microscopic findings. Group A: (1) Normal mitochondria and sarcolemma; (2) mitochondria are seen in myofibril and sarcomeres are neatly arranged. (Original magnification, both, ×20,000.) Group B: (3) Focal myofibrillar lysis and fragmentation, and vacuolar degeneration of mitochondria; (4) myocardial sarcolemma, swollen and fingerlike protrusions, and myocardial degeneration. (Original magnification, both, ×10,000.) The Annals of Thoracic Surgery 2010 89, 1518-1523DOI: (10.1016/j.athoracsur.2010.02.008) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions