Use of a multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay for the rapid identification of bacterial pathogens Y. Wang, F. Kong, G.L.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Studies which Can Be Used Sputum Analysis  Gram Stain  Fungal Smear  AFB Smear  Antibody Probes (DFA) Culture Antibody Testing.

GRAM POSITIVE & GRAM NEGATIVE BACTERIA

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

Distribution of phylogenetic groups, sequence type ST131, and virulence-associated traits among Escherichia coli isolates from men with pyelonephritis.

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

A. Bergman, D. Heimer, N. Kondori, H. Enroth

Validation of PCR–reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples A.M.C.

Gut bacterial microbiota and obesity

M.A.C. Broeren, Y. Maas, E. Retera, N.L.A. Arents

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated over a 2-year period in a Qatari hospital from multinational patients

Pneumococcal infection in adults: burden of disease

Clinical characteristics of Haemophilus influenzae meningitis in Denmark in the post- vaccination era T.I. Pedersen, M. Howitz, C. Østergaard Clinical.

Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae G. Tzanakaki,

Mycobacterial peritonitis: difference between non-tuberculous mycobacteria and Mycobacterium tuberculosis C.-C. Shu, J.-T. Wang, J.-Y. Wang, C.-J. Yu,

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

The microarray technology: facts and controversies

G. Zhao, H. Zhai, Q. Yuan, S. Sun, T. Liu, L. Xie

Validation of PCR–reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples A.M.C.

Development of a real-time PCR targeting the yidC gene for the detection of Mycoplasma hominis and comparison with quantitative culture C. Férandon,

Presence of the ica operon in clinical isolates of Staphylococcus epidermidis and its role in biofilm production V. Cafiso, T. Bertuccio, M. Santagati,

A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU P.G. Higgins, H. Wisplinghoff, O. Krut, H.

Comparison of the new InoDiag automated fluorescence multiplexed antigen microarray to the reference technique in the serodiagnosis of atypical bacterial.

Development and evaluation of a multiplex test for the detection of atypical bacterial DNA in community-acquired pneumonia during childhood J.A. Carrillo,

E. Cambau, M. Drancourt Clinical Microbiology and Infection

Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections

Characterizing non-linear effects of hospitalisation duration on antimicrobial resistance in respiratory isolates: an analysis of a prospective nationwide.

A.G.G. Lødeng, C. Ahlén, H. Lysvand, L.H. Mandal, O.J. Iversen

Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR T. Kallonen, J.

A multicentre analysis of epidemiology of the nosocomial bloodstream infections in Japanese university hospitals M. Nagao Clinical Microbiology and.

Pharmacodynamics of doxycycline

Inga Odenholt, Elisabeth Löwdin, Otto Cars

The changing epidemiology of bacteraemias in Europe: trends from the European Antimicrobial Resistance Surveillance System M.E.A. de Kraker, V. Jarlier,

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,

Pharmacodynamic properties of HMR 3004, a novel ketolide, on respiratory pathogens, enterococci and Bacteroides fragilis demonstrated by studies of time.

Low prevalence of methicillin-resistant Staphylococcus aureus with reduced susceptibility to glycopeptides in Belgian hospitals C. Nonhoff, O. Denis,

Development and evaluation of a multiplex test for the detection of atypical bacterial DNA in community-acquired pneumonia during childhood J.A. Carrillo,

Z. Wang, Z. Cui, Y. Li, T. Hou, X. Liu, Y. Xi, Y. Liu, H. Li, Q. He

Bacterial resistance—the clinical challenge

Comparative in vitro pharmacodynamics of BO-2727, meropenem and imipenem against Gram-positive and Gram-negative bacteria Inga Odenholt, Elisabeth Löwdin,

G. Kronvall Clinical Microbiology and Infection

Changes in macrolide resistance among respiratory pathogens after decreased erythromycin consumption in Taiwan P.-R. Hsueh, J.-M. Shyr, J.-J. Wu Clinical.

M. C. Alanin, K. G. Nielsen, C. von Buchwald, M. Skov, K. Aanaes, N

J. Buijs, A. S. M. Dofferhoff, J. W. Mouton, J. H. T. Wagenvoort, J. W

The selection of resistance to and the mutagenicity of different fluoroquinolones in Staphylococcus aureus and Streptococcus pneumoniae J.M. Sierra,

L. -N. Lee, C. -H. Chou, J. -Y. Wang, H. -L. Hsu, T. -H. Tsai, I. -S

T.M. File Clinical Microbiology and Infection

Long-term study of the frequency of Escherichia coli and Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases L. Romero, L. López,

Nosocomial listeria gastroenteritis in a newborn, confirmed by random amplification of polymorphic DNA H. Hof, R. Lampidis, J. Bensch Clinical Microbiology.

Antibacterial drug discovery in the 21st century

Community-acquired pneumonia: epidemiologic and clinical consideration

Distribution of phylogenetic groups, sequence type ST131, and virulence-associated traits among Escherichia coli isolates from men with pyelonephritis.

Pandemic lineages of extraintestinal pathogenic Escherichia coli

P.M. Hawkey Clinical Microbiology and Infection

Molecular epidemiology and virulence factors of pyogenic liver abscess causing Klebsiella pneumoniae in China Y. Luo, Y. Wang, L. Ye, J. Yang Clinical.

Comparative bactericidal activities of daptomycin, glycopeptides, linezolid and tigecycline against blood isolates of Gram-positive bacteria in Taiwan

Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds J.H. Melendez, Y.M. Frankel, A.T.

Catheter-related bloodstream infection caused by Enterococcus spp.

P. Kaltsas, S. Want, J. Cohen Clinical Microbiology and Infection

Clinical Microbiology and Infection

S. Figueiredo, L. Poirel, A. Papa, V. Koulourida, P. Nordmann

J.J. Keller, M.-C. Tsai, C.-C. Lin, Y.-C. Lin, H.-C. Lin

Modelling during an emergency: the 2009 H1N1 influenza pandemic

Assessment of the usefulness of performing bacterial identification and antimicrobial susceptibility testing 24 h a day in a clinical microbiology laboratory

Antibiotic overconsumption and resistance in Turkey

B.A. Cunha Clinical Microbiology and Infection

Mixed infection with Beijing and non-Beijing strains in pulmonary tuberculosis in Taiwan: prevalence, risk factors, and dominant strain J.-Y. Wang, H.-L.

G.C. Schito Clinical Microbiology and Infection

Enhanced isolation of Legionella species from composted material

The future of diagnostic bacteriology

Presentation transcript:

Use of a multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay for the rapid identification of bacterial pathogens Y. Wang, F. Kong, G.L. Gilbert, M. Brown, W. Gao, S. Yu, Y. Yang Clinical Microbiology and Infection Volume 14, Issue 2, Pages 155-160 (February 2008) DOI: 10.1111/j.1469-0691.2007.01890.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Detection of 24 bacterial reference strains using the multiplex PCR-based reverse line blot assay. DNA dilutions were as follows: Escherichia coli (500 ng - 5 pg), Bacteroides spp. (BS) (500 ng - 50 pg), Pseudomonas aeruginosa (PA) (5 μg - 50 pg). Probes are indicated on the y-axis, and bacteria are indicated on the x-axis. Numbers refer to reference strains of the following species: 1, Staphylococcus aureus; 2, Staphylococcus epidermidis; 3, Staphylococcus haemolyticus; 4, Staphylococcus hominis; 5, Staphylococcus saprophyticus; 6, Enterococcus faecalis; 7, Streptococcus pneumoniae; 8, Streptococcus pyogenes; 9, Streptococcus agalactiae; 10, Listeria monocytogenes; 11, Moraxella catarrhalis; 12, Neisseria meningitidis; 13, Bordetella pertussis; 14, Haemophilus influenzae; 15, H. influenzae type b; 16, Klebsiella pneumonia; 17, Legionella pneumophila; 18, E. coli; 19, P. aeruginosa; 20, Mycobacterium tuberculosis; 21, Acinetobacter spp.; 22, Bacteroides spp.; 23, Mycoplasma pneumoniae; and 24, Chlamydia pneumonia. Clinical Microbiology and Infection 2008 14, 155-160DOI: (10.1111/j.1469-0691.2007.01890.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions