M. Kanaa, M.J. Wright, J.A.T. Sandoe

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Presentation transcript:

Examination of tunnelled haemodialysis catheters using scanning electron microscopy M. Kanaa, M.J. Wright, J.A.T. Sandoe Clinical Microbiology and Infection Volume 16, Issue 6, Pages 780-786 (June 2010) DOI: 10.1111/j.1469-0691.2009.02952.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Scanning electron microscopy images of Staphylococcus epidermidis (a and b) and Escherichia coli (c and d) biofilm-containing polyvinyl chloride catheters at 1000× (a and c) and 25 000× (b and d). Clinical Microbiology and Infection 2010 16, 780-786DOI: (10.1111/j.1469-0691.2009.02952.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Bacteria (arrows, left) and possible bacterial micro-colonies (dashed arrows, right) on the endoluminal surface of an explanted Tesio catheter that was culture–positive for Streptococcus sanguinis (heparin lock). Clinical Microbiology and Infection 2010 16, 780-786DOI: (10.1111/j.1469-0691.2009.02952.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Endoluminal surface of an Arrow catheter removed from a patient with methicillin-sensitive Staphylococcus aureus catheter-related bloodstream infection showing a cluster of cocci buried within a fibrous-looking network (heparin lock). Clinical Microbiology and Infection 2010 16, 780-786DOI: (10.1111/j.1469-0691.2009.02952.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 4 Endoluminal surface of a negative-culture explanted Tesio catheter covered with coccus-shaped cells (heparin lock). Clinical Microbiology and Infection 2010 16, 780-786DOI: (10.1111/j.1469-0691.2009.02952.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 5 A culture-negative explanted Tesio catheter showing cocci and probably extracellular polymeric substances on the endoluminal surface (heparin lock). Clinical Microbiology and Infection 2010 16, 780-786DOI: (10.1111/j.1469-0691.2009.02952.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 6 Two culture-negative Tesio catheters covered by adherent biological material but no identifiable bacteria (both catheters were locked with EDTA). Clinical Microbiology and Infection 2010 16, 780-786DOI: (10.1111/j.1469-0691.2009.02952.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions