Resistance to infection of long-term cryopreserved human aortic valve allografts Viola Steffen, MD, Georg Marsch, MD, Karin Burgwitz, Christian Kuehn,

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Presentation transcript:

Resistance to infection of long-term cryopreserved human aortic valve allografts Viola Steffen, MD, Georg Marsch, MD, Karin Burgwitz, Christian Kuehn, MD, PhD, Omke E. Teebken, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 151, Issue 5, Pages 1251-1259 (May 2016) DOI: 10.1016/j.jtcvs.2015.11.029 Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Partition of 1 cryopreserved human allograft for microbiologic experiments and tissue examination. S. epid, Staphylococcus epidermis; S. aur, Staphylococcus aureus; E. coli, Escherichia coli; P. aeru, Pseudomonas aeruginosa; SEM, scanning electron microscopy. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1251-1259DOI: (10.1016/j.jtcvs.2015.11.029) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Bacteria (colony forming units/centimeter2) adherent to aortic wall or valve tissue after antibiotic treatment according to protocol A through C and after incubation with A, Staphylococcus epidermidis (S. epidermis) or B, Staphylococcus aureus (S. aureus). The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1251-1259DOI: (10.1016/j.jtcvs.2015.11.029) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Bacteria (colony forming units/centimeters2) adherent to aortic wall tissue after antibiotic treatment according to protocol A through C and after bacterial incubation. S. epidermis, Staphylococcus epidermis; S. aureus, Staphylococcus aureus; E. coli, Escherichia coli; P. aeruginosa, Pseudomonas aeruginosa. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1251-1259DOI: (10.1016/j.jtcvs.2015.11.029) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 A, Cryopreserved aortic wall tissue after antibiotic treatment according to protocol A through C and after incubation with A, control, B, Staphylococcus epidermis (S. epidermidis), C, Staphylococcus aureus (S. aureus), D, Escherichia coli (E. coli), or E, Pseudomonas aeroginosa (P. aeruginosa). The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1251-1259DOI: (10.1016/j.jtcvs.2015.11.029) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Cryopreserved aortic valve tissue after antibiotic treatment according to protocol A through C in A, control and after incubation with B, Staphylococcus epidermis (S. epidermidis) or C, Staphylococcus aureus (S. aureus). The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1251-1259DOI: (10.1016/j.jtcvs.2015.11.029) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Elongated Escherichia coli on cryopreserved aortic wall allograft. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1251-1259DOI: (10.1016/j.jtcvs.2015.11.029) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions