Repetitive progressive thermal preconditioning hinders thrombosis by reinforcing phosphatidylinositol 3-kinase/Akt-dependent heat-shock protein/endothelial.

Slides:

Advertisements

Similar presentations

Simvastatin ameliorates injury in an experimental model of lung ischemia-reperfusion Babu V Naidu, FRCS, Steven M Woolley, MRCS, Alexander S Farivar,

Advertisements

Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular.

Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis Yunjian Zhang, PhD, MD, Hanping Shi, PhD, MD, Wenjian.

Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.

Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells Wen-Jane Lee, PhD, Hsiu-Chung.

Crude extracts of Solanum lyratum protect endothelial cells against oxidized low-density lipoprotein-induced injury by direct antioxidant action Wei-Wen.

Jeanwan Kang, MD, Hassan Albadawi, MD, Patrick J. Casey, MD, Thomas A

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Volume 58, Issue 4, Pages (October 2000)

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia Geoffrey O. Ouma,

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis April E. Nedeau, MD,

Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells Wen-Jane Lee, PhD, Hsiu-Chung.

Crude extracts of Solanum lyratum protect endothelial cells against oxidized low-density lipoprotein-induced injury by direct antioxidant action Wei-Wen.

Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries Birgit Zatschler, Peter Dieterich, PhD, Bianca Müller,

Long-acting oral phosphodiesterase inhibition preconditions against reperfusion injury in an experimental lung transplantation model Eric S. Weiss, MD,

Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia- reperfusion injury Rachel J. Santora, MD, Mihaela L. Lie, MD, Dmitry.

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Bertrand Poussier, MD, Alfredo C

Dextran sulfate modulates MAP kinase signaling and reduces endothelial injury in a rat aortic clamping model Yara Banz, MD, PhD, Thusitha Gajanayake,

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries Jens W Fischer,

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model Jianping Dai, MD, PhD, Liliane.

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide.

The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Improvement of the cold storage of blood vessels with a vascular preservation solution. Study in porcine aortic segments Timo Wille, Herbert de Groot,

Circulating microparticles from patients with valvular heart disease and cardiac surgery inhibit endothelium-dependent vasodilation Li Fu, MD, Xiao-Xia.

Simvastatin ameliorates injury in an experimental model of lung ischemia-reperfusion Babu V Naidu, FRCS, Steven M Woolley, MRCS, Alexander S Farivar,

Progressive thermopreconditioning attenuates rat cardiac ischemia/reperfusion injury by mitochondria-mediated antioxidant and antiapoptotic mechanisms

Lipoplex gene transfer of inducible nitric oxide synthase inhibits the reactive intimal hyperplasia after expanded polytetrafluoroethylene bypass grafting

Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps Francesca Polito, PhD, Alessandra Bitto, MD, PhD, Mariarosaria.

Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model Ryan O. Lakin, MD, Weifei Zhu, PhD, Lindsay.

Iodinated contrast induced renal vasoconstriction is due in part to the downregulation of renal cortical and medullary nitric oxide synthesis Stuart.

Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis Yunjian Zhang, PhD, MD, Hanping Shi, PhD, MD, Wenjian.

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury

Protective effects of cold spinoplegia with fasudil against ischemic spinal cord injury in rabbits Hironori Baba, MD, Yoshihisa Tanoue, MD, PhD, Taketoshi.

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide.

Simvastatin pretreatment enhances ischemia-induced neovascularization and blood flow recovery in streptozotocin-treated mice Po-Hsun Huang, MD, PhD,

Ambient pressure upregulates nitric oxide synthase in a phosphorylated-extracellular regulated kinase– and protein kinase C–dependent manner Angela G.

The essential role of endothelial nitric oxide synthase activation in insulin-mediated neuroprotection against ischemic stroke in diabetes Shiang-Suo.

Polydeoxyribonucleotide (PDRN) restores blood flow in an experimental model of peripheral artery occlusive disease Alessandra Bitto, MD, Francesca Polito,

Expression and Function of Endothelial Nitric Oxide Synthase Messenger RNA and Protein Are Higher in Internal Mammary Than in Radial Arteries Guo-Wei.

Vikas Chander, PhD, Kanwaljit Chopra, PhD Journal of Vascular Surgery

Endothelial nitric oxide synthase affects both early and late collateral arterial adaptation and blood flow recovery after induction of hind limb ischemia.

Enhanced expression of glucose transporter-1 in vascular smooth muscle cells via the Akt/tuberous sclerosis complex subunit 2 (TSC2)/mammalian target.

Recanalization of arterial thrombus, and inhibition with β-radiation in a new murine carotid occlusion model: mRNA expression of angiopoietins, metalloproteinases,

Stuart I. Myers, MD, Li Wang, BS, Fang Liu, BS, Lori L. Bartula, BS

Single-dose rosuvastatin ameliorates lung ischemia–reperfusion injury via upregulation of endothelial nitric oxide synthase and inhibition of macrophage.

Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular.

Autophagy-mediated stress response in motor neurons after hypothermic spinal cord ischemia in rabbits Satoshi Fujita, MD, Masahiro Sakurai, MD, PhD,

Epigallocatechin-3-gallate is a potent phytochemical inhibitor of intimal hyperplasia in the wire-injured carotid artery Vicente Orozco-Sevilla, MD,

Dose-dependent neuroprotection of delta-opioid peptide [D-Ala2, D-Leu5] enkephalin on spinal cord ischemia-reperfusion injury by regional perfusion into.

Extracorporeal shock wave therapy ameliorates secondary lymphedema by promoting lymphangiogenesis Masayuki Kubo, PhD, Tao-Sheng Li, MD, PhD, Takahiro.

Gabriele Di Luozzo, MD, Jaya Bhargava, PhD, Richard J. Powell, MD

The effects of ginsenoside Rb1 on endothelial damage and ghrelin expression induced by hyperhomocysteine Zhiwei Xu, PhD, Taohua Lan, MD, Weikang Wu,

Blockade of poly(ADP-ribose) synthetase inhibits neutrophil recruitment, oxidant generation, and mucosal injury in murine colitis Basilia Zingarelli,

Thrombolysis for experimental deep venous thrombosis maintains valvular competence and vasoreactivity Jeffrey M. Rhodes, MD, Jae-Sung Cho, MD, Peter.

Michael A. Rosenbaum, MD, Keiko Miyazaki, MD, Linda M. Graham, MD

Ischemic preconditioning ameliorates spinal cord ischemia-reperfusion injury by triggering autoregulation Cheng-Loong Liang, MD, Kang Lu, MD, PhD, Po-Chou.

Autophagy-mediated stress response in motor neuron after transient ischemia in rabbits Hironori Baba, MD, Masahiro Sakurai, MD, PhD, Koji Abe, MD, PhD,

Deep vein thrombosis resolution is impaired in diet-induced type 2 diabetic mice Fatiha Bouzeghrane, PhD, Xiaochun Zhang, MD, BSc, Guylaine Gevry, BSc,

Role of Nitric Oxide Pathway in Placental Dysfunction Following Fetal Bypass Christopher Lam, BS, R. Scott Baker, BS, Jerri McNamara, CCP, Robert Ferguson,

Overexpression of endothelial nitric oxide synthase increases skeletal muscle blood flow and oxygenation in severe rat hind limb ischemia Lucy S Brevetti,

Exogenous nitric oxide modulates the systemic inflammatory response and improves kidney function after risk-situation abdominal aortic surgery Francisco.

Iodinated contrast induced renal vasoconstriction is due in part to the downregulation of renal cortical and medullary nitric oxide synthesis Stuart.

Suprarenal aortic clamping and reperfusion decreases medullary and cortical blood flow by decreased endogenous renal nitric oxide and PGE2 synthesis

Presentation transcript:

Repetitive progressive thermal preconditioning hinders thrombosis by reinforcing phosphatidylinositol 3-kinase/Akt-dependent heat-shock protein/endothelial nitric oxide synthase signaling Ping-Chia Li, PhD, Chih-Ching Yang, MD, PhD, Shih-Ping Hsu, MD, PhD, Chiang-Ting Chien, PhD Journal of Vascular Surgery Volume 56, Issue 1, Pages 159-170 (July 2012) DOI: 10.1016/j.jvs.2011.11.062 Copyright © 2012 Society for Vascular Surgery Terms and Conditions

Fig 1 A, The setup for thermal preconditioning (TP) or progressive thermal preconditioning (PTP) in the conscious rat is demonstrated. The bath water was maintained at 42°C. TP was performed at line 3 for 15 minutes. PTP was performed progressively at line 1 for 5 minutes, line 2 for 5 minutes, and line 3 for 5 minutes. B, The colonic temperature (CT) change is recorded in control (Con), PTP, and TP groups. C, The respective areas under the curve (AUC; mean time × CT) of three groups are displayed. A significant (P < .05) increase in total energy transfer is found in TP and PTP groups compared with the Con group. TP has a higher total energy transfer than the PTP group. The error bars show the standard error of the mean. D, TP increases heart rate but PTP does not. E, TP but not PTP increased final mean blood pressure (MBP). F, TP and PTP significantly increased final CT when compared to Con group. *P < .05 vs Con; #P < .05 vs TP with saline treatment. Journal of Vascular Surgery 2012 56, 159-170DOI: (10.1016/j.jvs.2011.11.062) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

Fig 2 A, The temporal responses (0.5-4 hours) of the carotid arterial expression of phosphorylated endothelial nitric oxide synthase (p-eNOS), p-Akt, glucose-regulated protein (GRP78), and heat-shock protein (Hsp) 70 after TP, 1-PTP, and 3-PTP treatment are demonstrated. Enhanced p-eNOS expression was recognized after 2 hours of 1-PTP treatment, 0.5 hour of 3-PTP, and 0.5 hour of TP treatment. Enhanced p-Akt was found after 1 hour of 1-PTP, 1 hour of 3-PTP, and 0.5 hour of TP stimulation. Increased GRP78 expression was identified after 2 hours of 1-PTP, 1 hour of 3-PTP, and 0.5 hour of TP treatment. Elevated Hsp70 expression was noted after 2 hours of 1-PTP, 0.5 hour of 3-PTP, and 0.5 hour of TP. Expressions of p-eNOS and p-Akt were similar between 3-PTP and TP after 4 hours of treatment, whereas GRP78 and Hsp70 expressions were highly expressed in the TP group compared with 3-PTP or 1-PTP groups. The expression in p-eNOS, p-Akt, GRP78, and Hsp70 was mildly enhanced in the 1-PTP group but was highly enhanced in the 3-PTP and TP groups. B, After 0.5 to 4 hours of 1-PTP, 3-PTP, or TP treatment, blood reactive oxygen species (ROS) levels were significantly increased vs control (Con) group. The increased ROS levels were significantly less in the 1-PTP and 3-PTP groups compared with the TP group. *P < .05 vs respective TP group. PTP, Progressive thermal preconditioning; 1-PTP, one cycle of PTP; 3-PTP, three cycles of PTP. Journal of Vascular Surgery 2012 56, 159-170DOI: (10.1016/j.jvs.2011.11.062) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

Fig 3 A, The responses of carotid arterial blood flow to ferric chloride (FeCl3) stimulation in seven groups of animals are demonstrated. FeCl3 stimulation significantly reduced carotid arterial blood flow in all groups between 1250 and 2600 seconds. The time for reducing carotid arterial blood flow to zero (time to occlusion [TTO]) was significantly extended in 1-PTP-24 (b), 1-PTP-72 (c), 3-PTP-24 (f), 3-PTP-72 (g), TP-24 (d) and TP-72 (e) groups vs control (Con) group (a). B, The TTO values in 3-PTP-24 and 3-PTP-72 were not significantly different than those in TP-24 and TP-72 groups. TTO levels in 1-PTP-24 and 1-PTP-72 were significantly less than those in 3-PTP or TP groups. *P < .05 vs Con without any treatment; #P < .05 vs 1-PTP-24. The error bars show the standard error of the mean. PTP, Progressive thermal preconditioning; TP, thermal preconditioning; see the Table for explanation of 1-PTP-24, 1-PTP-72, 3-PTP-24, 3-PTP-72, TP-24, and TP-72 experimental groups. Journal of Vascular Surgery 2012 56, 159-170DOI: (10.1016/j.jvs.2011.11.062) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

Fig 4 The degree of oxidative stress chemokine (C-X3-C motif) ligand 1 (CX3CL1), thrombus size, and infiltrated leukocytes in FeCl3-induced vascular injury is analyzed in response to 900 seconds of ferric chloride (FeCl3) stimulation in the experimental groups. A-D, Vascular oxidative stress (2′,7′-dichlorodihydrofluorescein diacetate [DCFDA] green fluorescence), cellular DNA damage (propidium iodide [PI], red fluorescence), (E-H) CX3CL1 expression (brown color), (I-L) thrombus size, and (M-P) leukocyte number (arrows) by hematoxylin and eosin stain in the carotid arteries of seven groups. A, There were no marked stain of DCFDA and (E) CX3CL1, (I) no thrombus formation, and (M) almost rare leukocyte stain in control (Con) artery without FeCl3 injury. A marked stain in (B) DCFDA and (F) CX3CL1, (J) thrombus formation, and (N) increased leukocyte number are found in Con artery with FeCl3 injury. 3-PTP-72 or TP-72 treatment decreased FeCl3-induced (C and D) DCFDA and (G and H) CX3CL1, (K and L) thrombus size, and (O and P) leukocyte number in these arterial sections. Data analysis showed that FeCl3-increased thrombus size was significantly suppressed in (Q) 1-PTP, 3-PTP, or TP groups, whereas increased leukocyte number in thrombus was depressed by (R) 1-PTP, 3-PTP, and TP treatment. 3-PTP was more significantly efficient than 1-PTP in reducing thrombus size and leukocyte infiltration. 3-PTP has a similar effect vs TP treatment. *P < .05 vs Con without any treatment; #P < .05 vs Con with FeCl3; aP < .05 vs 1-PTP group. PTP, Progressive thermal preconditioning; TP, thermal preconditioning; see the Table for explanation of 3-PTP-72 and TP-72 experimental groups. Journal of Vascular Surgery 2012 56, 159-170DOI: (10.1016/j.jvs.2011.11.062) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

Fig 5 A, Vascular intercellular adhesion molecule 1 (ICAM-1), 3-nitrotyrosine (3-NT), and 4-hydroxynonenal (4-HNE) expressions of the carotid arteries in control (Con), 1-PTP-72, 3-PTP-72, and TP-72 are shown in response to 900 seconds of ferric chloride (FeCl3) stimulation. Enhanced vascular ICAM-1 (b-e), 4-HNE (g-j), and 3-NT (l-o) expressions were found in FeCl3-treated carotid arteries compared with noninjured arteries (a, f, and k). B, These enhanced expressions were significantly suppressed in the 1-PTP-72, 3-PTP-72, and TP-72 groups compared with the Con group. There was no difference in these stains between 3-PTP-72 and TP-72 groups. The arterial malondialdehyde (MDA) concentration after the FeCl3-induced injury was significantly increased in the Con, 1-PTP-72, 3-PTP-72, and TP-72 groups compared with non–FeCl3-treated groups. C, The elevated MDA was significantly depressed in 1-PTP-72, 3-PTP-72, and the TP-72 groups. 3-PTP-72 and TP-72 were more efficient than 1-PTP-72 in reducing MDA level. D, The baseline level of vascular plasminogen activator inhibitor type 1 (PAI-1) and (E) tissue plasminogen (t-PA) activity was similar in Con, 1-PTP-72, 3-PTP-72, and TP-72 groups. FeCl3 treatment significantly increased carotid arterial PAI-1 and t-PA activity. The elevated PAI-1 and t-PA activity was significantly depressed in the 1-PTP-72, 3-PTP-72, and TP-72 groups. *P < .05 vs Con without any treatment; #P < .05 vs Con with FeCl3; aP < .05 vs 1-PTP. PTP, Progressive thermal preconditioning; TP, thermal preconditioning; see the Table for explanation of the 1-PTP-72, 3-PTP-72, and TP-72 groups. Journal of Vascular Surgery 2012 56, 159-170DOI: (10.1016/j.jvs.2011.11.062) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

Fig 6 A, Immunoblots demonstrate original heat-shock protein (Hsp)70, phosphorylated Akt (p-Akt), and phosphorylated endothelial nitric oxide synthase (p-eNOS). B, Hsp70, p-Akt, and p-eNOS expressions were significantly enhanced in the carotid arteries of 1-PTP-24, 1-PTP-72, 3-PTP-24, 3-PTP-72, TP-24, and TP-72 groups than in those in the control (Con) group. C, A significantly increased NO amount in the carotid arterial samples was found in all PTP or TP groups vs the Con group. D and E, Administration of intraperitoneal LY294002, quercetin, and N(G)-nitro-l-arginine methyl ester hydrochloride (L-NAME) significantly suppressed the enhanced Hsp70, p-Akt, and p-eNOS expressions between the 3-PTP-72 and TP-72 groups. F, Intraperitoneal LY294002, quercetin, and L-NAME also significantly reduced the carotid time to total occlusion (TTO) level to a similar level between 3-PTP-72 and TP-72 groups. *P < .05 vs Con without any treatment; #P < .05 vs respective 1-PTP-24, 3-PTP-24, or TP-24 group; aP < .05 vs 1-PTP-72. See the Table for an explanation of the 1-PTP-24, 1-PTP-72, TP-24, and TP-72 groups. Journal of Vascular Surgery 2012 56, 159-170DOI: (10.1016/j.jvs.2011.11.062) Copyright © 2012 Society for Vascular Surgery Terms and Conditions