Chromatography Introduction and Plumbing Harris Ch. 23

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Presentation transcript:

Chromatography Introduction and Plumbing Harris Ch. 23 November 12, 2018

Time = tm “Dead time”

Time = tm “Dead time” time No separation!

Retention time t’r Mobile phase Stationary phase Time for solvent molecule is the same as before: tm Time for analyte molecule is different: tr Retention time tm t’r Adjusted retention time: tr time

If you have many molecules Retention factor: Time in stationary phase Distribution coefficient Time in mobile phase If you have many molecules or

t’r(1) Selectivity factor Separation factor Relative retention t’r(2) Mobile phase Stationary phase Time for solvent molecule is the same as before: tm Time for analyte molecules is different: tr(1) and tr(2) tm t’r(1) Selectivity factor Separation factor Relative retention tr t’r(2) time

Distribution coefficient Retention factor k: Separation factor:

Plate Number Plate Height W = 4s L - length of column Number of theoretical plates: Plate Number W = 4s L - length of column Height of theoretical plate is: H = L / N = L  s2 / tr2 Plate Height

The van Deemter equation Zone broadening: The van Deemter equation H - plate height u - flow rate

A = 0

B

van Deemter plot