Protective Effects of Pulsatile Flow During Cardiopulmonary Bypass

Slides:

Advertisements

Similar presentations

Squamous Cell Carcinoma of the Pericardium Found During Coronary Bypass Surgery of Possible Cutaneous Origin Alan P. Kypson, MD, Curtis A. Anderson,

Advertisements

Intraaortic Counterpulsation During Cardiopulmonary Bypass Impairs Distal Organ Perfusion Steinar Lundemoen, MD, Venny Lise Kvalheim, MD, PhD, Øyvind.

Risk Index for Postoperative Acute Kidney Injury After Valvular Surgery Using Cardiopulmonary Bypass Takashi Yamauchi, MD, PhD, Shigeru Miyagawa, MD,

The STS National Database

Elaine E. Tseng, MD, Malcolm V. Brock, MD, Mary S. Lange, MA, Juan C

Rodrigo A. S. Sardenberg, MD, Clovis Pinto, MD, Cynthia A

The Myocardial Protective Effects of a Moderate-Potassium Blood Cardioplegia in Pediatric Cardiac Surgery: A Randomized Controlled Trial Yang Liu, MD,

Collision Tumor of Esophagus: Report of Three Cases

Cholesterol Granuloma: A Rare Benign Rib Tumor

Alexander S. Farivar, MD, Anton S. McCourtie, MRCS, Brendan C

Thoracic Pseudoaneurysm Caused By Malignant Lymphoma

Invited Commentary The Annals of Thoracic Surgery

Total Arch Replacement Under Normothermic Beating Heart Surgery

Burdens Without Blessings: Peer Reviewers Get No Respect

Primary Chordoma of the Lung

John D. Rutkoski, MD, Beth A. Schrope, MD, Benjamin E. Lee, MD

Neutrophil Elastase Inhibitor, Sivelestat, Attenuates Acute Lung Injury After Cardiopulmonary Bypass in the Rabbit Endotoxemia Model Fuminori Wakayama,

Mesenchymal Cystic Hamartoma of the Lung

Philip W. Smith, MD, Hongkun Wang, PhD, Leo M. Gazoni, MD, K

Total Liquid Ventilation Reduces Lung Injury in Piglets After Cardiopulmonary Bypass Lijun Jiang, MD, Qiang Wang, MD, Yinglong Liu, MD, Ming Du, MD, Xiangdong.

Susanne Lison, MD, Michael Spannagl, MD, PhD, Wulf Dietrich, MD, PhD

Nodular Lymphoid Hyperplasia of the Lung: A Very Rare Disease Entity

Late Presentation of a Right Bochdalek Hernia With Intrathoracic Liver, Gallbladder, Intestines and Omentum With Right Lower Lobe Hypoplasia Mohammad.

Pleural Metastasis of Osteosarcoma

Percutaneous Tissue-Engineered Pulmonary Valved Stent Implantation

Primary Pulmonary Lymphoma

Origins of the Cardiothoracic Surgery Network (CTSNet)

Who Do You Say You Are? The Annals of Thoracic Surgery

Josephina Haunschild, MD, Isabel N

Use of Amiodarone After Major Lung Resection

Thymic and Mediastinal Lymph Node Metastasis of Colon Cancer

Abel Gómez-Caro, MD, Pedro Arguis, MD The Annals of Thoracic Surgery

Outcomes and Costs for Major Lung Resection in the United States: Which Patients Benefit Most From High-Volume Referral? Elliot Wakeam, MD, MPH, Joseph.

Fatty Infiltration of an Aortic Valve

Ivan Ricardo Argueta-Morales, MD, Hamish M

Esophageal Actinomycoses Mimicking Malignancy

Effects of perfusion mode on regional and global organ blood flow in a neonatal piglet model Akif Ündar, PhD, Takafumi Masai, MD, Shuang-Qiang Yang,

Benjamin C. Sun, MD, Timothy A. Dickinson, CCP, Eric A

Open, Video-Assisted Thoracic Surgery, and Robotic Lobectomy: Review of a National Database Michael Kent, MD, Thomas Wang, PhD, Richard Whyte, MD, Thomas.

Embolized Methyl Methacrylate to the Right Atrium After Kyphoplasty

Localized Amyloid Light-Chain Amyloidosis and Extramedullary Plasmacytoma of the Mitral Valve Aurélien Roumy, MD, Laurence de Leval, MD, Lars Niclauss,

Hisashi Ishikura, MD, PhD, Suguru Kimura, MD, PhD

Editorial Board, January 2010

Giant Cardiac Cavernous Hemangioma

Bradford B. Smith, MD, Matthew A

Sarah C. Huen, MD, Chirag R. Parikh, MD, PhD

Mitral Valve Necrosis After Cardiac Surgery Using Gelatin-Resorcinol-Formaldehyde Glue Yuki Tanaka, MD, Tadashi Kitamura, MD, PhD, Yurie Miyata, MD,

Red Blood Cell Transfusion Is Associated With Troponin Release After Elective Off- Pump Coronary Artery Bypass Surgery Fausto Biancari, MD, PhD, Eeva-Maija.

Completely Thrombosed Tricuspid Pouch Mimicking a Cardiac Tumor

Vascular Leiomyoma of the Pulmonary Artery

Minocycline-Induced Hyperpigmentation Mimicking Aortic Dissection

Histopathologic consequences of hyperglycemic cerebral ischemia during hypothermic cardiopulmonary bypass in pigs Brendan P Conroy, MD, Marjorie R Grafe,

Chondroid Syringoma: A Rare Tumor of the Chest Wall

Treatment of Plastic Bronchitis

The Annals of Thoracic Surgery

Pulmonary Embolectomy for Acute Massive Pulmonary Embolism

Mature Cystic Teratoma With Malignant Transformation to Adenocarcinoma

“Transitions” for Cardiothoracic Surgeons Now Listed on the Web:

Posttraumatic Main Left Bronchial Rupture

Catheter-Based Endoscopic Bypass Grafting: An Experimental Feasibility Study Stephan Jacobs, MD, David Holzhey, MD, Hubert Stein, BS, BME, Friedrich W.

Successful use of argatroban as a heparin substitute during cardiopulmonary bypass: heparin-induced thrombocytopenia in a high-risk cardiac surgical patient

Ross Rudolph, MD, Michael R. Smith, MD, Guy P. Curtis, MD, PhD

Beating Heart Off-Pump Myocardial Revascularization in an Infant

Mark I. Block, MD The Annals of Thoracic Surgery

Late Consequences of a Much Earlier Decision

An Assay to Monitor Bivalirudin Levels on Cardiopulmonary Bypass

The Impact of New Technology on Cardiothoracic Surgical Practice

Karl A. Bocchieri, BS, CCP, S. Jacob Scheinerman, MD, L

A Small Alveolar Adenoma Resected by Thoracoscopic Surgery

Myoung-Gwi Ryou, PhD, Devin C

Presentation transcript:

Protective Effects of Pulsatile Flow During Cardiopulmonary Bypass Aida Salameh, MD, PhD, Lydia Kühne, MD, Maria Grassl, Maria Gerdom, MD, Sandy von Salisch, PhD, Marcel Vollroth, MD, Farhad Bakhtiary, MD, PhD, Friedrich-Wilhelm Mohr, MD, Ingo Dähnert, MD, Stefan Dhein, MD The Annals of Thoracic Surgery Volume 99, Issue 1, Pages 192-199 (January 2015) DOI: 10.1016/j.athoracsur.2014.07.070 Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 (A) Hematoxylin and eosin (H&E) staining of hippocampus CA1 and CA3 regions of control piglets and piglets after non-pulsatile and pulsatile cardiopulmonary bypass (CPB), respectively. Bar graphs depict the number of cells (in %) with pericellular edema. (B) H&E-staining of kidney glomeruli and tubules and of liver lobules of control piglets and piglets after non-pulsatile and pulsatile CPB, respectively. For the kidney, bar graphs depict the gap between Bowman capsule and the capillary convolute (in μm, glomeruli) and the number of tubular cells (in %) with vacuoles. For the liver, bar graphs depict the number of edematous cells per liver lobule. (* = significant differences to control ; # = significant differences to non-pulsatile CPB; p < 0.05.) (C) Original H&E-staining of hippocampus (HC), kidney, and liver (magnification 200×). Arrows indicate pericellular edema (hippocampus), the gap between Bowman capsule and the capillary convolute, and vacuolated cells of tubules (kidney), respectively. The Annals of Thoracic Surgery 2015 99, 192-199DOI: (10.1016/j.athoracsur.2014.07.070) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 (A) Hypoxia-inducible factor-1α (HIF1α) staining of hippocampus CA1 and CA3 regions of control piglets and piglets after non-pulsatile and pulsatile cardiopulmonary bypass (CPB), respectively. Bar graphs depict the number of positive cells nuclei (in %). (* = significant differences to control ; # = significant differences to non-pulsatile CPB; p < 0.05.) (B) HIF1α staining of kidney glomeruli and tubules and of liver lobules of control piglets and piglets after non-pulsatile and pulsatile CPB, respectively. For the kidney, bar graphs depict the number of positive cell nuclei (in %) of glomeruli and proximal tubules, respectively. For the liver, bar graphs depict the number of positive cell nuclei per liver lobule. (Significant differences to control are indicated by asterisks; p < 0.05). (C) Original HIF1α-staining of hippocampus (HC), kidney and liver (magnification 200×). Arrows indicate cell nuclei positive for HIF1α (stained in red). The Annals of Thoracic Surgery 2015 99, 192-199DOI: (10.1016/j.athoracsur.2014.07.070) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 (A) Apoptosis-inducing factor (AIF) staining of hippocampus CA1 and CA3 regions of control piglets and piglets after non-pulsatile and pulsatile cardiopulmonary bypass (CPB), respectively. Bar graphs depict the number of positive cells nuclei (in %). (* = significant differences to control ; # = significant differences to non-pulsatile CPB; p < 0.05.) (B) AIF-staining of kidney glomeruli and tubules and of liver lobules of control piglets and piglets after non-pulsatile and pulsatile CPB, respectively. For the kidney, bar graphs depict the number of positive cell nuclei (in %) of glomeruli and proximal tubules, respectively. For the liver, bar graphs depict the number of positive cell nuclei per liver lobule. (Significant differences to control are indicated by asterisks; p < 0.05). (C) Original AIF-staining of hippocampus (HC), kidney, and liver (magnification 200×). Arrows indicate cell nuclei positive for AIF (stained in red). The Annals of Thoracic Surgery 2015 99, 192-199DOI: (10.1016/j.athoracsur.2014.07.070) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig. 4 (A) Poly-ADP-ribose (PAR) staining of hippocampus CA1 and CA3 regions of control piglets and piglets after non-pulsatile and pulsatile cardiopulmonary bypass (CPB), respectively. Bar graphs depict the number of positive cells nuclei (in %). (* = significant differences to control; # = significant differences to non-pulsatile CPB; p < 0.05.) (B) PAR staining of kidney glomeruli and tubules, and of liver lobules of control piglets and piglets after non-pulsatile and pulsatile CPB, respectively. For the kidney, bar graphs depict the number of positive cell nuclei (in %) of glomeruli and proximal tubules, respectively. For the liver, bar graphs depict the number of positive cell nuclei per liver lobule. (* = significant differences to control; # = significant differences to non-pulsatile CPB; p < 0.05.) (C) Original PAR-staining of hippocampus (HC), kidney, and liver (magnification 200×). Arrows indicate cell nuclei positive for PAR (red = HC and liver; dark red = kidney). The Annals of Thoracic Surgery 2015 99, 192-199DOI: (10.1016/j.athoracsur.2014.07.070) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 5 (A) Nitrotyrosine-staining of hippocampus CA1 and CA3 regions of control piglets and piglets after non-pulsatile and pulsatile cardiopulmonary bypass (CPB), respectively. Bar graphs depict the number of positive cells (in %). (* = significant differences to control; # = significant differences to non-pulsatile CPB; p < 0.05.) (B) Nitrotyrosine staining of kidney glomeruli and tubules and of liver lobules of control piglets and piglets after non-pulsatile and pulsatile CPB, respectively. For the kidney, bar graphs depict the number of positive cells (in %) of proximal tubules. In the glomeruli no nitrotyrosine-positive cells could be detected. For the liver, bar graphs depict the number of positive cells per liver lobule. (* = significant differences to control; # = significant differences to non-pulsatile CPB; p < 0.05.) (C) Original nitrotyrosine staining of hippocampus (HC), kidney, and liver (magnification 200×). Arrows indicate cells positive for nitrotyrosine (purple = HC; red = kidney and liver). Note the absent nitrotyrosine staining in the glomeruli. The Annals of Thoracic Surgery 2015 99, 192-199DOI: (10.1016/j.athoracsur.2014.07.070) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions