Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a/ ESC clones (B) were injected into C57BL/6N blastocysts.

Slides:

Advertisements

Similar presentations

DNA Chips Attach DNA to tiny spots on glass slides (i.e., chip). Hybridize fluorescently-labeled DNA probes to chip. Detect hybridization to different.

Advertisements

H. influenzae genes differentially regulated during growth in complex media and during lung infection in the presence of corticosteroids AVenn diagram.

Hyp mice show hypertension and increased NCC expression and phosphorylation A–ESerum intact Fgf23 concentration (A, n = 8–9, Student's t‐test, *P =

Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD

TZD enhances cisplatin sensitivity through suppression of endotrophin‐mediated EMT, fibrosis and angiogenesis.A,B.Schematic diagram for allografts (A),

GLUT3 is a direct target of TCF4/β‐catenin

Volume 86, Issue 1, Pages (July 1996)

Heatmaps of the gene mutation distributions in oncogene‐signaling blocks for six representative cancer types. Heatmaps of the gene mutation distributions.

The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.

Globin Gene Expression Is Reprogrammed in Chimeras Generated by Injecting Adult Hematopoietic Stem Cells into Mouse Blastocysts Hartmut Geiger, Stefanie.

Relationship between Genotype and Phenotype

Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD

AAV8‐hAAT‐FGF21 treatment improves liver fibrosis

The absence of COL6 in PyMT mice sensitizes tumours to cisplatin treatment.A.Eleven week old FP635/PyMT and FP635/PyMT/COL6−/− mice were given cisplatin.

Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

CAR T cell dose and percentages of CAR‐positive cells within CAR T cell products CAR T cell dose and percentages of CAR‐positive cells within CAR T cell.

by Hiromi Gunshin, Carolyn N. Starr, Cristina DiRenzo, Mark D

Metabolic profile of Tfe3 KO mice

Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.

Volume 29, Issue 1, Pages (April 2014)

Increased IFNγ producing CD4+ T cells in the spleen drives the enhanced splenic IFNγ on 6 dpi during concurrent co‐infection Increased IFNγ producing CD4+

EGFL7 affected tumor growth and reduced overall survival

Fig. 4. Antitumor efficacy of ERY974 against various cancer types.

SPP1 and TNC promote chemoresistance

Mouse SCNT ESCs Have Lower Somatic Mutation Load Than Syngeneic iPSCs

Relationship between Genotype and Phenotype

The upstream region of MUC5AC bound by SPDEF is required for the expression of MUC5AC in human lung cancer cells The upstream region of MUC5AC bound by.

SPRY2 deficiency mediates CRPC

T22‐GFP‐H6‐FdU prevents metastasis in the M5 patient‐derived model in a CXCR4‐dependent manner T22‐GFP‐H6‐FdU prevents metastasis in the M5 patient‐derived.

Stat3C/C mice are more prone to Th17 differentiation. A

Dietary administration of DHEA reduces jet‐lag

Molecular and clinical characterization of AAV2/8‐TBG‐h

Neutralizing endotrophin activity with monoclonal antibodies sensitizes tumours to cisplatin treatment.A.Pieces of tumours from PyMT mice were implanted.

Nec‐1 inhibits the phosphorylation and aggregation of tau

High frequency and unique tumor‐initiating cells (TICs) in Pten/p53‐deficient mice Flow cytometry profiles of indicated tumors with the CD24 and CD49f.

Hdac3 deletion decreases AKT phosphorylation and tumor growth in Pten knockout prostate cancer Hdac3 deletion decreases AKT phosphorylation and tumor growth.

Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+ tumors Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

FMRP is highly expressed in human breast cancer and distal metastasis FMRP expression on human TMAs containing normal and multi‐tumour tissues. FMRP is.

Deletion of Crebbp in the GC cooperates with BCL2 deregulation to promote lymphomagenesis. Deletion of Crebbp in the GC cooperates with BCL2 deregulation.

Volume 4, Issue 1, Pages (January 2015)

MiR‐146b induces 3T3‐L1 adipocyte differentiation qRT‐PCR analysis of miR‐146b during 3T3‐L1 differentiation (n = 4). miR‐146b induces 3T3‐L1 adipocyte.

GPR120 deficiency alters the expression of genes involved in glucose and lipid metabolism in BAT GPR120 deficiency alters the expression of genes involved.

Malkiel A. Cohen, Styliani Markoulaki, Rudolf Jaenisch

Combined targeting of PCa AR and CCL2/CCR2 axis suppresses tumour growth and reduces metastasis in a xenograft mouse PCa model Proliferation assay of TRAMP‐C1.

MiR‐34a‐5p functionally contributes to arrested lung alveolarization in response to hyperoxia miR‐34a‐5p functionally contributes to arrested lung alveolarization.

Both the number and the OX40L expression level of bronchiolar progenitors were increased by influenza infection. Wild‐type mice were intratracheally infected.

High CDK6 expression predicts poor prognosis in EOC patients

miR‐205 modulates tumor cell sensitivity to MET‐TKIs

The Mucinous Lung Tumor Signature is highly enriched in colorectal, stomach, pancreatic, lung (adenocarcinoma; LUAD), and breast cancers The Mucinous Lung.

Dual‐recombinase fluorescence lineage tracing of EMT in KPF (FSF‐KrasG12D/+;Trp53frt/+;Pdx1‐Flp);αSMA‐Cre;R26Dual mice Dual‐recombinase fluorescence lineage.

Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts.

Tissue‐specific differences in the levels of CoQ9, CoQ10, and DMQ9, and in the DMQ9/CoQ9 ratio after β‐RA treatment in Coq9R239X mice Tissue‐specific differences.

Volume 7, Issue 2, Pages (August 2016)

Genomewide profiling of chromatin accessibility in prostate cancer specimens Genomewide profiling of chromatin accessibility in prostate cancer specimens.

Many of the diseases associated with high coevolution scores share genetic components. Many of the diseases associated with high coevolution scores share.

Figure 5. Tissue uptake of circulating 125I-β2-m.

Nilotinib inhibits DDR1 invasive activity of patient‐derived CRC cells

CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair depigmentation CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair.

Expression of p110γ isoform in macrophages promotes tumor growth and angiogenesis. Expression of p110γ isoform in macrophages promotes tumor growth and.

Nanog-Independent Reprogramming to iPSCs with Canonical Factors

Expression of PKCδ in islets and other tissues of control and PKCδKN-overexpressing mice. Expression of PKCδ in islets and other tissues of control and.

Volume 86, Issue 1, Pages (July 1996)

Rapid target gene validation in complex cancer mouse models using re‐derived embryonic stem cells In vivo imaging of a invCAG‐Luc;Rb1F/F;Trp53F/F chimeric.

Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model in vivo. Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model.

Effect of the crystal form and solid dispersion preparations of KRN633 on the growth of human tumor xenografts in mice. Effect of the crystal form and.

Fig. 6 Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9 in vivo. Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9.

Osteoactivin is overexpressed in aggressively bone metastatic 4T1 subpopulations versus weakly bone metastatic breast cancer populations. Osteoactivin.

Proliferation of TA3-Ha and TA3-St cells in vitro and in vivo.

In vivo shRNA screening strategy.

Presentation transcript:

Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts and scored for their chimeric contribution. Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts and scored for their chimeric contribution. All ESC clones gave reasonable numbers of chimeric animals relative to the implanted embryos (supplementary Table S1) and, as expected, most of the chimeras were males as we exclusively used male ESC clones. ■ male, female, n.d. C, DComparison between chimeric contribution estimated on basis of coat‐color versus genetic chimerism, tested in various tissues. Southern blot analysis was performed with a probe that distinguishes between a wild‐type Trp53 allele or the floxed Trp53 allele reflecting the contribution by the host ESCs or cultured ESCs, respectively (example in supplementary Fig S3). Controls are wild‐type spleen (0% chimerism expected) and F1 offspring of chimeras (50% chimerism expected). (C) Genetic chimerism of Rb1F/F;Trp53F/F chimeras with coat color chimerism ranging from 70 to 100% (average 84%, n = 7). (D) Genetic chimerism of Nf2F/F;Trp53F/F;Cdkn2a*/* chimeras with coat color chimerism ranging from 85 to 100% (average 95%, n = 4). ESurvival curves of Rb1F/F;Trp53F/F mice intratracheally injected with Ad5‐Cre. Black line, conventional mice; red line, chimeras. FSurvival curves of Nf2F/F;Trp53F/F;Cdkn2a*/* mice intrathoracically injected with Ad5‐Cre. Black line: conventional mice; Red line: chimeras. GTypical example of a neuroendocrine carcinoma (Small Cell Lung Cancer) in the lung (left panel) and a metastatic lesion in the liver (right panel). HTypical example of a mesotheliomatous lesion in the thoracic cavity. Tumor cells are either spindle sarcomatoid cells (left panel) or vacuolated epithelioid cells (right panel). Ivo J Huijbers et al. EMBO Mol Med. 2014;6:212-225 © as stated in the article, figure or figure legend