O2 CO2 RESPIRATORY ENZYMES ATP Catalaze H202 ENERGY Superoxide dismutase O2 H202 Amal Ali ALmousa 140 mic 30/03/1440

Anaerobies Aerobes Obligate aerobes Microaerophiles Facultative anaerobes Microaerophiles Obligate aerobes Obligate anaerobes Aerotolrant anaerobes Amal Ali ALmousa 140 mic 30/03/1440

Microaerophiles Facultative anaerobes Obligate anaerobes Aerotolrant anaerobes Obligate aerobies Amal Ali ALmousa 140 mic 30/03/1440

Obligate anaerobes in lab Amal Ali ALmousa 140 mic 30/03/1440

Balanced growth Growth cycle: Lag phase Log phase Stationary phase Death phase The Growth Curve Amal Ali ALmousa 140 mic 30/03/1440

Measuring Growth 1-Cell Count Direct Measurement Direct Microscopic Count In direct Measurement colony counting hemocytometer Amal Ali ALmousa 140 mic 30/03/1440

3-Measurement of enzymatic activity In direct Measurement 2-Mass determination Direct Measurement weighed (wet mas). dried weighed( dry mass In direct Measurement Turbidity ( 3-Measurement of enzymatic activity In direct Measurement Amal Ali ALmousa 140 mic 30/03/1440

Factors affecting microbial growth 1-Physical factors 2-Chemical factors 3-Biological factors Amal Ali ALmousa 140 mic 30/03/1440

1-Physical factors Temperature O2 pH Osmotic Pressure radiation Moisture Desication Surface tension atmospheric pressure Amal Ali ALmousa 140 mic 30/03/1440

1-Temperature Minimum growth temperature Optimum growth temperature Maximum growth temperature Amal Ali ALmousa 140 mic 30/03/1440

2-Oxygen concentration slid 2 3- pH value Acidophilus :pH 0 and 5.5 Neutrophilus : pH 5.5 and 8 Alkalophileus :pH 8 – 11.5 Amal Ali ALmousa 140 mic 30/03/1440

:4-Osmotic Pressure A-Hypotonic B-Hypertonic Plasmolysis C-Isotonic Amal Ali ALmousa 140 mic 30/03/1440

UV rays, Ionizing radiation ))5- radiation 6-Moisture (Water activity) 7-Desiccation Amal Ali ALmousa 140 mic 30/03/1440

9- atmospheric pressure 8-Surface tension 9- atmospheric pressure Amal Ali ALmousa 140 mic 30/03/1440

2-Chemical factors 1-Germistatic agents 2-Germicidal agents Amal Ali ALmousa 140 mic 30/03/1440

Microbes group RELATIONS 3-Biological factors Microbes group RELATIONS Amal Ali ALmousa 140 mic 30/03/1440