CAFs enhance the acinar forming capacity of SOX2oe TUM622 cells and suppress dysplasia. CAFs enhance the acinar forming capacity of SOX2oe TUM622 cells.

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CAFs enhance the acinar forming capacity of SOX2oe TUM622 cells and suppress dysplasia. CAFs enhance the acinar forming capacity of SOX2oe TUM622 cells and suppress dysplasia. (A) Quantification of spheroids formed from Vector and SOX2oe cells as monoculture/coculture with CAFs. Each column represents triplicates. Error bars represent SD. (B) CAFs induce the formation of acinar-like structures when in coculture with SOX2oe cells. Both Vector and SOX2oe spheroids become invasive when they are in close proximity with CAFs. (Scales bars, 100 μm.) (C) Immunofluorescence of SOX2oe monoculture as well as CAF cocultures stained for Vimentin (cytoplasmic)/p63 (nuclear), E-Cadherin, and ITGA6. Anti-vimentin and anti-p63 antibodies were stained with the same secondary antibody (green). (D) Immunofluorescence of SOX2oe monoculture as well as CAF cocultures stained for Vimentin, SOX2, and Phalloidin. (Scale bars, 50 μm.)‏ Shuang Chen et al. PNAS doi:10.1073/pnas.1803718115 ©2018 by National Academy of Sciences