TUM622 is capable of forming acinar-like structures in 3D ECM

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TUM622 is capable of forming acinar-like structures in 3D ECM TUM622 is capable of forming acinar-like structures in 3D ECM. (A) Representative bright-field images of spheroids derived from single NSCLC cells in 3D ECM culture between days 8 and 12. TUM622 is capable of forming acinar-like structures in 3D ECM. (A) Representative bright-field images of spheroids derived from single NSCLC cells in 3D ECM culture between days 8 and 12. (Top) Cell lines (TUM622, TUM426, TUM449, and TUM110) are derived from either patient tumors or the corresponding PDX. (Bottom) Established cell lines of LUSC (H520), LUAD (HCC2935), and immortalized bronchial epithelial cells (BEAS2B and NHBE). (Scale bars, 50 μm.) (B) Time course images of TUM622 cell cultured in Matrigel over a 10-d period. (Top) Bright-field images. (Bottom) Green is calcein, marking live cells; red is ethidium bromide, marking dead cells. (Scale bars, 100 μm.) (C) Quantification of acini number (right y axis, red) and average size of acini (left y axis, blue) plated in triplicate in a 24-well plate over 23 d in culture. Error bars represent SD. (D) (Left) Immunofluorescence of TUM622 acini stained with apical−basal cell polarity markers Golgi enzyme GM130 (green, apical) and ITGA6 (CD49f, basal, red), (Right) in comparison with β-catenin staining (green). (Scale bars, 50 μm.)‏ Shuang Chen et al. PNAS doi:10.1073/pnas.1803718115 ©2018 by National Academy of Sciences