Expression of stem/progenitor-specific TFs

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Expression of stem/progenitor-specific TFs Expression of stem/progenitor-specific TFs. (A–D) Maximum projection z-stacks showing expression of conserved TFs in the midgut. Expression of stem/progenitor-specific TFs. (A–D) Maximum projection z-stacks showing expression of conserved TFs in the midgut. Background signal was subtracted using the remove outliers function (Fiji) and brightness/contrast increased for clarity. DAPI (blue), esg-lacZ (red), and TF (green) for: (A) Sox21a-GFP–tagged fosmid, (B) Sox100B-GFP trap, (C) jumu-GFP protein trap, and (D) apt-GFP protein trap. (Scale bars, 20 μm.) (E–G) Quantification of TF reporter overlap with (E) esg-lacZ, (n ≥ 10 guts), (F) Delta-lacZ (n ≥ 9 guts), (G) Su(H)-lacZ (n ≥ 9 guts), and (H) prospero staining (n ≥ 7 guts). David P. Doupé et al. PNAS 2018;115:48:12218-12223 ©2018 by National Academy of Sciences