Simulations capture the experimental force profiles for mutant I27 domains. Simulations capture the experimental force profiles for mutant I27 domains.

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Simulations capture the experimental force profiles for mutant I27 domains. Simulations capture the experimental force profiles for mutant I27 domains. (A) Mutated residues in I27 (sticks). (B–D) Experimental (red) force profiles and calculated ones from the full kinetic scheme (blue) for (B) I27[L58A], (C) A-strand deletion mutant I27[−A], and (D) I27[M67A]. I27[M67A] (Sim HP) represents a simulation in which hydrophobic interactions between I27[M67A] and ribosome proteins uL23/uL29 are included. Experimental force profiles for nonfolding mutants that contain an additional W34E mutation are shown as open red squares. The rmsd of the fFL between experiment and simulation for I27[L58A] and I27[−A] are 0.07 and 0.08, respectively. For I27[M67A], the fFL rmsd is 0.07 between experiment and simulation (Sim HP). Pengfei Tian et al. PNAS 2018;115:48:E11284-E11293 ©2018 by National Academy of Sciences