Introduction to Gel Electrophoresis
Outline How to prepare a gel How to micropipet Practice setting up electrophoresis Discussion viewing of gel In a later lab you will view and photograph your results
Agarose is weighed out
Agarose is diluted and boiled in buffer solution
Agarose solution is poured into gel holder This is a “comb”
Notice the “sample wells” Agarose cools and solidifies Notice the “sample wells” comb
Next: How to pipet into the sample well
Select either a 100 or 200 ul micropipet from your lab station (1000 ul= 1 ml) Set at 25 ul Place on a yellow tip
Push plunger to “first stop” Place tip in solution Aspirate sample by releasing plunger
Carefully place the tip of the micropipet just inside the well Dispense solution by pushing to second stop Release tip by “ejection button”
Gel and samples are subjected to electric current
Samples migrate through the gel at different rates Negative electrode Positive electrode
View your DNA samples with UV light Bio 22
Photograph the results