A Comparative Study of DNA Complexation with Mg(II) and Ca(II) in Aqueous Solution: Major and Minor Grooves Bindings R. Ahmad, H. Arakawa, H.A. Tajmir-Riahi

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A Comparative Study of DNA Complexation with Mg(II) and Ca(II) in Aqueous Solution: Major and Minor Grooves Bindings R. Ahmad, H. Arakawa, H.A. Tajmir-Riahi Biophysical Journal Volume 84, Issue 4, Pages 2460-2466 (April 2003) DOI: 10.1016/S0006-3495(03)75050-4 Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 1 FTIR spectra (top three curves) and difference FTIR spectra [(DNA solution+metal ion)−(DNA solution)] (two bottom curves) of the uncomplexed calf thymus DNA and its Mg and Ca complexes in aqueous solution at pH=7.4 with various cation/DNA(P) molar ratios in the region of 1800–600cm−1. Biophysical Journal 2003 84, 2460-2466DOI: (10.1016/S0006-3495(03)75050-4) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 2 Intensity ratio variations for several DNA in-plane vibrations at 1717 (guanine, thymine), 1663 (thymine, guanine, adenine, cytosine), 1609 (adenine), 1492 (cytosine, guanine), and 1222cm−1 (PO2 stretch) as a function of Mg and Ca concentrations (different cation-DNA(P) molar ratios). Biophysical Journal 2003 84, 2460-2466DOI: (10.1016/S0006-3495(03)75050-4) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 3 Plots for cation concentrations versus increase of peak height for the Mg and Ca complexes. Incubation of constant concentration of DNA (1.25mM of phosphate) with various concentrations of cations were carried out in 10mM Tris-HCl and 4mM NaCl (pH 7.4), and the mixtures were subjected to capillary electrophoresis. The increase of peak height in cation-DNA was determined by subtracting the peak height of the free DNA from each cation-DNA adduct. Biophysical Journal 2003 84, 2460-2466DOI: (10.1016/S0006-3495(03)75050-4) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 4 Scatchard plot for the Mg-DNA complexes. Biophysical Journal 2003 84, 2460-2466DOI: (10.1016/S0006-3495(03)75050-4) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 5 Scatchard plot for the Ca-DNA complexes. Biophysical Journal 2003 84, 2460-2466DOI: (10.1016/S0006-3495(03)75050-4) Copyright © 2003 The Biophysical Society Terms and Conditions