Marked difference between adults and children in Bordetella pertussis DNA load in nasopharyngeal swabs Y. Nakamura, K. Kamachi, H. Toyoizumi-Ajisaka,

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Presentation transcript:

Marked difference between adults and children in Bordetella pertussis DNA load in nasopharyngeal swabs Y. Nakamura, K. Kamachi, H. Toyoizumi-Ajisaka, N. Otsuka, R. Saito, J. Tsuruoka, T. Katsuta, N. Nakajima, K. Okada, T. Kato, Y. Arakawa Clinical Microbiology and Infection Volume 17, Issue 3, Pages 365-370 (March 2011) DOI: 10.1111/j.1469-0691.2010.03255.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Detection limit for linear calibration of IS481 real-time PCR. Serial dilutions of Bordetella pertussis Tohama DNA were subjected to the real-time PCR. Data are the mean ± SD for three independent experiments. Clinical Microbiology and Infection 2011 17, 365-370DOI: (10.1111/j.1469-0691.2010.03255.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Comparison of Bordetella pertussis DNA loads in nasopharyngeal swabs among children and adults with pertussis. (a) Children (infant and older children, n = 40) versus adults (n = 19). (b) Infants (n = 14) versus older children (n = 26). The DNA samples (2 μL) were subjected to IS481 real-time PCR. Horizontal bars indicate mean Ct values. Clinical Microbiology and Infection 2011 17, 365-370DOI: (10.1111/j.1469-0691.2010.03255.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions