The Intracellular Highway

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Presentation transcript:

The Intracellular Highway Protein synthesis and Transport GLOWING MARMOSETS CLASS 2 2/13/10 S. BACHAR AND R. ZACHARIAH

Each define an organelle  tie it all together at the end. Organelles

Transcription and Translation What does the m in mRNA stand for? Why? Transcription and Translation

Protein Targeting Protein Targeting

Activity! You are a scientist and you notice that most proteins that end up in the rough endoplasmic reticulum have the same sequence on one end of the protein. Design an experiment to prove your hypothesis – either the signal sequence is enough to get any protein into the ER or not.

Membrane Proteins How do they get there?

Finding Order: Temperature Sensitive Mutants

Finding Order: Temperature Sensitive Mutants

Jelly Fish! AEQUOREA VICTORIA

10 - 50 µm 100 - 200 µm 1 - 2 cm WHAT IS MICROSCOPY?

Bright Field and stains

Fluorescence

Using Antibodies

GFP-Tagging

Organelle Staining

SEM/TEM