The Intracellular Highway Protein synthesis and Transport GLOWING MARMOSETS CLASS 2 2/13/10 S. BACHAR AND R. ZACHARIAH
Each define an organelle tie it all together at the end. Organelles
Transcription and Translation What does the m in mRNA stand for? Why? Transcription and Translation
Protein Targeting Protein Targeting
Activity! You are a scientist and you notice that most proteins that end up in the rough endoplasmic reticulum have the same sequence on one end of the protein. Design an experiment to prove your hypothesis – either the signal sequence is enough to get any protein into the ER or not.
Membrane Proteins How do they get there?
Finding Order: Temperature Sensitive Mutants
Finding Order: Temperature Sensitive Mutants
Jelly Fish! AEQUOREA VICTORIA
10 - 50 µm 100 - 200 µm 1 - 2 cm WHAT IS MICROSCOPY?
Bright Field and stains
Fluorescence
Using Antibodies
GFP-Tagging
Organelle Staining
SEM/TEM