Increased fetal abortion rate in autoimmune thyroid disease is related to circulating TPO autoantibodies in an autoimmune thyroiditis animal model  Yin Lau Lee, Ph.D., Hang Pong Ng, Ph.D., Kam Shing Lau, Wei Min Liu, Ph.D., Wai Sum O, Ph.D., William S.B. Yeung, Ph.D., Annie W.C. Kung, F.R.C.P.  Fertility and Sterility  Volume 91, Issue 5, Pages 2104-2109 (May 2009) DOI: 10.1016/j.fertnstert.2008.07.1704 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Uteri with glutathione-S-transferase in complete Freund adjuvant (GST-CFA) (left) and recombinant mouse thyroid peroxidase (rmTPO) (right) immunization. One resorption site (arrow) in the left horn is seen in the rmTPO-immunized mouse. Fertility and Sterility 2009 91, 2104-2109DOI: (10.1016/j.fertnstert.2008.07.1704) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Representative photomicrographs showing binding of anti–thyroid peroxidase (TPO) antibody on the preimplantation embryo. Immunocytochemistry of anti-TPO under confocal microscope (×400). Anti-TPO (fluorescein isothiocyanate) bound to the membrane of the blastomere of 8-cell to morula-stage embryos (A). No signal was found without the addition of anti-TPO (C). (B and D) Phase contrast images. Propidium iodide–stained nucleus shown in red. Fertility and Sterility 2009 91, 2104-2109DOI: (10.1016/j.fertnstert.2008.07.1704) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions