Steven C. Verberckmoes, Marc E. De Broe, Patrick C. D'Haese 

Slides:



Advertisements
Similar presentations
Volume 63, Issue 3, Pages (March 2003)
Advertisements

Volume 67, Issue 2, Pages (February 2005)
Volume 61, Issue 1, Pages (January 2002)
Volume 66, Issue 1, Pages (July 2004)
Figure 3. Effects of SMFs on calcium content and osteoblastic or cementoblastic gene expression in a cell line of human PDLCs and cementoblasts. (A, B)
Volume 64, Issue 3, Pages (September 2003)
Volume 63, Issue 3, Pages (March 2003)
Effects of suppressing intrarenal angiotensinogen on renal transforming growth factor- β1 expression in acute ureteral obstruction  Gyu-Tae Shin, Wook-Hwan.
Volume 61, Issue 1, Pages (January 2002)
Volume 67, Issue 3, Pages (March 2005)
Volume 68, Issue 6, Pages (December 2005)
IL-2–mediated apoptosis of kidney tubular epithelial cells is regulated by the caspase-8 inhibitor c-FLIP  Caigan Du, Qiunong Guan, Ziqin Yin, Robert.
Volume 60, Issue 4, Pages (October 2001)
Volume 90, Issue 3, Pages (September 2016)
Volume 69, Issue 4, Pages (February 2006)
G.-T. Shin, D.R. Kim, J.-E. Lim, H. Yim, H. Kim  Kidney International 
Volume 80, Issue 7, Pages (October 2011)
Uremia-related vascular calcification: More than apatite deposition
Human renal epithelial cells produce the long pentraxin PTX3
Human mesangial cells express inducible macrophage scavenger receptor
Volume 69, Issue 8, Pages (April 2006)
Volume 62, Issue 4, Pages (October 2002)
Volume 63, Issue 6, Pages (June 2003)
Volume 67, Issue 5, Pages (May 2005)
Volume 60, Issue 5, Pages (November 2001)
Pulmonary Fibroblasts Induce Epithelial Mesenchymal Transition and Some Characteristics of Stem Cells in Non-Small Cell Lung Cancer  Yasushi Shintani,
Volume 65, Issue 5, Pages (May 2004)
Volume 67, Issue 4, Pages (April 2005)
Volume 26, Issue 1, Pages (January 2018)
Volume 67, Issue 4, Pages (April 2005)
Volume 68, Issue 1, Pages (July 2005)
Volume 65, Issue 4, Pages (April 2004)
Volume 64, Issue 5, Pages (November 2003)
Volume 140, Issue 3, Pages e8 (March 2011)
Evidence for the dysregulated expression of TWIST1, TGFβ1 and SMAD3 in differentiating osteoblasts from primary hip osteoarthritis patients  D.D. Kumarasinghe,
Volume 67, Issue 5, Pages (May 2005)
Volume 62, Issue 5, Pages (November 2002)
Volume 63, Issue 2, Pages (February 2003)
Hsueh Yang, Gabrielle Curinga, Cecilia M. Giachelli 
Volume 54, Issue 2, Pages (August 1998)
1,25-dihydroxyvitamin D3 inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression  Yingjian Li, Bradley C.
Volume 61, Issue 5, Pages (May 2002)
Volume 137, Issue 4, Pages (October 2009)
Volume 78, Issue 6, Pages (September 2010)
Akito Maeshima, Yoshihisa Nojima, Itaru Kojima  Kidney International 
Immunoglobulin light chains in uremia
Protective effect of vitamin C on 8-hydroxy-2′-deoxyguanosine level in peripheral blood lymphocytes of chronic hemodialysis patients  Der-Cherng Tarng,
Volume 66, Issue 1, Pages (July 2004)
Volume 61, Issue 6, Pages (June 2002)
Volume 62, Issue 5, Pages (November 2002)
Strontium causes osteomalacia in chronic renal failure rats
Volume 64, Issue 6, Pages (December 2003)
Volume 59, Issue 3, Pages (March 2001)
Volume 61, Issue 6, Pages (June 2002)
A.K.I. Kuroki, Masayuki Iyoda, Takanori Shibata, Tetsuzo Sugisaki 
Tohru Umekawa, Nasser Chegini, Saeed R. Khan, Ph.D. 
Volume 58, Issue 2, Pages (August 2000)
Angiotensin II stimulates Pax-2 in rat kidney proximal tubular cells: Impact on proliferation and apoptosis  Shao-Ling Zhang, Jun Guo, Babak Moini, Julie.
Volume 56, Issue 5, Pages (November 1999)
Volume 64, Issue 6, Pages (December 2003)
Effect of asymmetric dimethylarginine on osteoblastic differentiation
Zinc deficiency further increases the enhanced expression of endothelin-1 in glomeruli of the obstructed kidney  Hiroyuki Yanagisawa, Kazuaki Moridaira,
Calcium channel blockers, either amlodipine or mibefradil, ameliorate renal injury in experimental diabetes  Guorong Ma, Terri J. Allen, Mark E. Cooper,
Volume 64, Issue 1, Pages (July 2003)
Cultured human glomerular mesangial cells express the C5a receptor
J. Martin, T. Bowen, R. Steadman  Kidney International 
Volume 61, Issue 2, Pages (February 2002)
Triptolide is a potent suppressant of C3, CD40 and B7h expression in activated human proximal tubular epithelial cells  Yuzhi Hong, Wuding Zhou, Ke Li,
Volume 65, Issue 6, Pages (June 2004)
Hyperphosphatemia-induced nanocrystals upregulate the expression of bone morphogenetic protein-2 and osteopontin genes in mouse smooth muscle cells in.
Presentation transcript:

Dose-dependent effects of strontium on osteoblast function and mineralization  Steven C. Verberckmoes, Marc E. De Broe, Patrick C. D'Haese  Kidney International  Volume 64, Issue 2, Pages 534-543 (August 2003) DOI: 10.1046/j.1523-1755.2003.00123.x Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 1 (A) Light microscopic photograph of a partially mineralized nodule of a control primary osteoblast culture. Upper panel, Grey lines enclose the total nodule area while white lines contain the mineralized area of the total nodule as measured by the digital image analysis. Subtracting the mineralized area from the total nodule area yields the unmineralized area (magnification, ×100). EPXMA spectrum of a mineralized nodule and (lower panel) matching scanning electron microscopic image (bar represents 10μ). The 10/6 Ca/P ratio (middle panel) confirms the deposited mineral to be Ca hydroxyapatite. (B) Upper panel, FTIR spectra of pure Ca hydroxyapatite; middle panel, pulverized animal bone; lower panel, mineralized primary rat osteoblast culture. Peaks at 1030, 605, and 560cm-1 are characteristic for Ca hydroxyapatite. EPXMA is electron probe x-ray micro analysis; FTIR is Fourier transform infra red. Kidney International 2003 64, 534-543DOI: (10.1046/j.1523-1755.2003.00123.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 2 (A) Nodule area expressed as mm2/cm2 quantified at the end of the experiment. Black bars represent the mineralized area and open bars represent the unmineralized area of the nodules. For the quantitative representation of the degree of mineralization calculated as the percentage of mineralized nodule area see Results. *P < 0.05 vs. control. (B) Unstained cultures showing the mineralized nodules of the corresponding cultures at the end of the incubation period (magnification, ×1). (C) Area under the time-Ca incorporation curve. Data are expressed as μg Ca incorporated per well (corrected for DNA content) and presented as the mean ± SD calculated from 6-pooled wells of 3 different cultures. *P < 0.05 vs. control. (D) Area under the time-concentration curves for alkaline phosphatase expressed in U/L calculated from 6-pooled wells of 3 different cultures, each of them measured in duplicate and corrected for DNA content. Data are presented as mean ± SD. *P < 0.05 vs. control. Kidney International 2003 64, 534-543DOI: (10.1046/j.1523-1755.2003.00123.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 3 BrdU-incorporation in the different groups under study in function of time. No significant differences between the different groups were found. Kidney International 2003 64, 534-543DOI: (10.1046/j.1523-1755.2003.00123.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 4 RT-PCR analysis demonstrating the mRNA expression of ALP, BMP-2, BSP, CBFa-1, and OPN. RT-PCR was performed in duplo on mRNA isolates from three independent cultures (N = 6) treated with various doses of Sr. Quantitative data of the expression levels of the different genes are expressed as mean ± SEM and corrected for the mRNA expression of GAPDH. Abbreviations are: RT-PCR, reverse transcription-polymerase chain reaction; ALP, alkaline phosphatase; BMP-2, bone morphogenic protein-2; BSP, bone sialoprotein; CBFa-1, core binding factor alpha 1; OPN, osteopontin; GAPDH, glyceraldehydes-3-phosphate-dehydrogenase. Kidney International 2003 64, 534-543DOI: (10.1046/j.1523-1755.2003.00123.x) Copyright © 2003 International Society of Nephrology Terms and Conditions