Peixoto et al, Supplementary Figure 4.1 A(top)

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Peixoto et al, Supplementary Figure 4.1 A(top) ROP17-HA ROP19-HA ROP20-HA ROP23-HA Figure S 4.1. ROPK Localization. A, Most ROPK proteins localize to the rhoptries and parasitophorous vacuole, as detected by co-localization of antibodies to HA-tagged antigen (green) and native ROP2/3/4 (red); blue = DAPI (host nuclei are visible in some images). B, ROP21 and ROP22 do not co-localize with rhoptry proteins, but are secreted into the parasitophorous vacuole and are observed within the host cytoplasm late during infection (Fig. 3). ROP25-HA ROP39-HA ROP40-HA anti-ROP2 merge anti-HA phase

Peixoto et al, Supplementary Figure 4.1 B(top) ROP21-HA ROP22-HA Figure S 4.1. ROPK Localization. A, Most ROPK proteins localize to the rhoptries and parasitophorous vacuole, as detected by co-localization of antibodies to HA-tagged antigen (green) and native ROP2/3/4 (red); blue = DAPI (host nuclei are visible in some images). B, ROP21 and ROP22 do not co-localize with rhoptry proteins, but are secreted into the parasitophorous vacuole and were also observed within the host cytoplasm late during infection (Fig. 3). phase anti-HA anti-ROP2 merge