Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine.

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Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine Molecular Diagnostics Lina Zhang, Guanglin Cui, Zongzhe Li, Haoran Wang, Hu Ding, Dao Wen Wang The Journal of Molecular Diagnostics Volume 15, Issue 5, Pages 600-606 (September 2013) DOI: 10.1016/j.jmoldx.2013.04.005 Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Representative genotype results of rs4244285 by each method. A and E: Sanger sequencing. Red and black boxes indicate rs4244285 (G>A) allele and unexpected heterozygous variant, respectively. B: TaqMan allelic discrimination assay. The three groups indicate three different genotypes of rs4244285 (red, wild type; green, heterozygous; and blue, homozygous variant; black, negative controls). C and D: HRMA normalized melting curves and difference plot. Samples were classified into four clusters indicating three different genotypes of rs4244285 (red, wild type; green, heterozygous; blue, homozygous variant) and unexpected heterozygous variant (gray curves). The Journal of Molecular Diagnostics 2013 15, 600-606DOI: (10.1016/j.jmoldx.2013.04.005) Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions