Terse ImageJ Manual When you startup ImageJ, the main console will appear on your screen. The relevant features of the main console for this activity.

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Presentation transcript:

Terse ImageJ Manual When you startup ImageJ, the main console will appear on your screen. The relevant features of the main console for this activity are summarized below. File – managers opening, saving, closing of image files Edit – undo button, cut, copy, and paste Analyze – measure toolbar, set measurements Image – adjust picture setting, properties, and crop pictures

Loading an Image 1. Click File > Open (Crtl+O) 2. Browse for image, select image, click Open 3. Selected image will appear in new window

Calibrate an Image 1. Click Image > Properties 2. Enter image calibration factor for pixel width, heigth, & depth. Change unit of length to microns 3. Check input and click OK

Image Measurements 1. Length Measurements: Right click line icon for length measurement options 2. Click and drag over feature of interest. Click Analyze > Measure (Crtl +M) 3. Measurement will appear in new Results window Feature

Image Measurements Cont. 4. Area Measurements: Choose from following options on Main Console Freehand Polygonal Area Elliptical Area Rectangular Area 5. Outline area of interest. Right click to close outline. 6. Repeat Steps 2- 3 to record area measurement.

This completes the Terse ImageJ Manual This completes the Terse ImageJ Manual. If more program features are needed, but consult official ImageJ manual online. Link to ImageJ Documentation http://rsbweb.nih.gov/ij/docs/index.html