AZD7762 abrogated the G2 checkpoint in a novel hollow fiber assay.

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AZD7762 abrogated the G2 checkpoint in a novel hollow fiber assay. AZD7762 abrogated the G2 checkpoint in a novel hollow fiber assay. A, schematic diagram illustrating the hollow fiber assay. HCT116 cells were loaded into prepared fibers, incubated in medium, and then incubated in topotecan-containing medium for 18 h. Fibers were implanted into mice and AZD7762 was delivered i.v. Thirty hours later, fibers were removed from the mice and cells from the fibers were fixed with ethanol and stained with propidium iodide. Cell cycle profiles were determined by fluorescence-activated cell sorting analysis. B, fluorescence-activated cell sorting profiles of cells treated with vehicle control, topotecan alone, or topotecan followed by AZD7762. The percentage of cells in the G1 and G2 phase and calculated odds ratio values are listed. C, dose-dependent increase in the odds ratio following treatment with AZD7762. Sonya D. Zabludoff et al. Mol Cancer Ther 2008;7:2955-2966 ©2008 by American Association for Cancer Research