Two color superresolution MFM PALM/STORM images of yeast cells containing α-tubulin–tdEos- and Con A–Alexa 647-labeled cell wall. Two color superresolution.

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Two color superresolution MFM PALM/STORM images of yeast cells containing α-tubulin–tdEos- and Con A–Alexa 647-labeled cell wall. Two color superresolution MFM PALM/STORM images of yeast cells containing α-tubulin–tdEos- and Con A–Alexa 647-labeled cell wall. The red–yellow scale codes the z positions of Alexa 647 detections, whereas the blue–white scale corresponds to tdEos. (Left) Cells are shown in the xy view with color-coded depth. (Scale bars: 1 μm.) (Center) A tilted view reveals the mitotic spindle going through the neck of the bud during division. (Axes lengths: 1 μm.) (Right) Surface rendering of tdEos localizations in blue–white. Here, the color code represents molecule density. Snap shots present the budding yeast at different stages of cell division. (Scale bars and axes lengths: 1 μm.) (A) Metaphase cell with a short intranuclear spindle not oriented toward the neck. (B) Cell entering anaphase with a spindle beginning to penetrate through the neck opening. (C) Late anaphase/telophase cell with the mitotic spindle going through the neck of the bud (Movie S6). (D) Cell at the end of telophase with a disintegrating spindle breaking up between mother and daughter cells. (E) Postmitotic G1 cells without spindles. Bassam Hajj et al. PNAS 2014;111:49:17480-17485 ©2014 by National Academy of Sciences