Phosphatase activity associated with Themis is due to its association with Shp1. Phosphatase activity associated with Themis is due to its association.

Slides:

Advertisements

Similar presentations

Volume 16, Issue 1, Pages (January 2009)

Advertisements

Volume 56, Issue 5, Pages (November 1999)

Figure Legend: From: Role of ErbB2 in Corneal Epithelial Wound Healing

A novel SHP-1/Grb2–dependent mechanism of negative regulation of cytokine-receptor signaling: contribution of SHP-1 C-terminal tyrosines in cytokine signaling.

JNK is activated by Wnt5a.

Posttranslational regulation of ETV5 and c-JUN in neurons.

Calcium regulates ERK nuclear association, but not its activation.

FAM49B controls T cell activation by regulating cytoskeleton remodeling. FAM49B controls T cell activation by regulating cytoskeleton remodeling. (A) J.FAM49B.

DHEA(S) and Allo activated transcription factors NF-κB and CREB in serum-deprived PC12 cells. DHEA(S) and Allo activated transcription factors NF-κB and.

IP-MS identifies FAM49B interacting protein Rac.

Lipopolysaccharide Activates Caspase-1 (Interleukin-1–Converting Enzyme) in Cultured Monocytic and Endothelial Cells by Ralf R. Schumann, Claus Belka,

TGF-β1 induces ILK activity in renal tubular epithelial cells.

DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived PC12 cells. DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived.

PGAM5 dephosphorylates ASK1.

Takashi Tanaka, Michelle A. Soriano, Michael J. Grusby Immunity

HDAC3, but not HDAC2 is in complex with tau protein.

PGAM5 is a protein Ser/Thr phosphatase that activates ASK1.

p110-free p85 is present in MEFs and certain murine tissues.

P110-free p85 is recruited to PDGFRs with higher affinity than the p85-p110 dimers at low levels of receptor activation. p110-free p85 is recruited to.

Despite a relatively small amount of PI3Kβ being associated with activated PDGFRs, it generates a more substantial proportion of the PIP3 that accumulates.

P85α and p110δ, but not other subunits, interact preferentially in vivo. p85α and p110δ, but not other subunits, interact preferentially in vivo. (A) Compares.

Phosphatase activity of PGAM5 is required for activation of ASK1.

Antisense oligonucleotides against the Bcl-2 transcript reversed the cytoprotective effects of DHEA(S) and Allo on serum-deprived PC12 cells. Antisense.

PGAM5 associates with and activates ASK1.

Interaction with Snail1 is required for LSD1 recruitment to the E‐cadherin promoter in vivo. Interaction with Snail1 is required for LSD1 recruitment to.

Pirh2 promotes p73 ubiquitination in vivo.

Themis acts to increase phosphorylation of Shp1.

Thymic phenotype of Themis−/− Shp1 cKO mice is similar to that of Themis−/− mice. Thymic phenotype of Themis−/− Shp1 cKO mice is similar to that of Themis−/−

Phosphatase activity associated with Themis and Shp1 in response to different affinity peptide ligands. Phosphatase activity associated with Themis and.

Themis interacts with Shp2 when Shp1 is genetically deleted.

by Mi-Ae Kang, Su-Young Yun, and Jonghwa Won

Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes.

Requirements of src family kinase activity associated with CD45 for myeloma cell proliferation by interleukin-6 by Hideaki Ishikawa, Naohiro Tsuyama, Saeid.

Volume 56, Issue 5, Pages (November 1999)

Western blot of Aβ42-specific antibody compared with 4G8 antibody.

Nuclear Arp3 mediates formation of TCR-induced nuclear actin filaments

DAO enhances DNA damage–induced ROS accumulation, cooperating with PRODH. DAO enhances DNA damage–induced ROS accumulation, cooperating with PRODH. (A)

Arachidonic acid induces ERK activation via Src SH2 domain association with the epidermal growth factor receptor L.D. Alexander, Y. Ding, S. Alagarsamy,

MED12 interacts with TDRD3 in a CARM1-dependent fashion.

Christopher L. Kepley, PhDa, John C. Cambier, PhDb, Penelope A

Volume 22, Issue 11, Pages (March 2018)

Siqi Lin, Claudia Cicala, Andrew M Scharenberg, Jean-Pierre Kinet Cell

TIMP-2 Mediated Inhibition of Angiogenesis

CDK4/6 knockdown causes upregulation of MYC, GLS1, and P‐mTOR and downregulation of HIF‐1α CDK4/6 knockdown causes upregulation of MYC, GLS1, and P‐mTOR.

Arp2/3-mediated formation of nuclear actin networks is essential for CD4+ T cell effector functions. Arp2/3-mediated formation of nuclear actin networks.

Fig. 1 LB100 and LB102 specifically inhibit PP2A phosphatase activity and the growth of BCR-ABL+ cells. LB100 and LB102 specifically inhibit PP2A phosphatase.

Sarcomere length-dependence of the M3 reflection at rest (A) and at the plateau of an isometric tetanus (B). Sarcomere length-dependence of the M3 reflection.

DMT1 expression in the mesencephalon of MPTP-intoxicated mice.

Regulation of PP6 phosphatase activity by Plk1 in vitro.

Anu Cherukuri, Paul C. Cheng, Hae Won Sohn, Susan K. Pierce Immunity

Activation of PKR by the PBM decoy peptide.

AMRs in the turnover of secreted proteins.

Accumulation of asialoglycoproteins, agalactoglycoproteins, and Man-terminated N-glycans during blood protein aging. Accumulation of asialoglycoproteins,

AMPK directly increases mTORC2 catalytic activity.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

FeTTPS blocks tyrosine nitration of TrkA receptor and restores TrkA-Y490 phosphorylation in RGCs. A: Peroxynitrite (PN) increased TrkA tyrosine nitration.

Time-dependent effects of IL-6 on tyrosine phosphorylation of IRS-1.

SusG LES is required for efficient packing into OMVs

Effect of the selective pharmacological inhibitors used.

Transgenic c-Maf mediates upregulation of type 2 cytokines in NOD CD4, but not CD8, cells. Transgenic c-Maf mediates upregulation of type 2 cytokines in.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation. DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation.

Mapping the Pirh2 and p73 interaction sites.

Effect of G9a on the expression of GSH synthesis enzymes.

HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. HNSCC and NSCLC cell lines display differential.

Activation of the Lck Tyrosine Kinase Targets Cell Surface T Cell Antigen Receptors for Lysosomal Degradation Ugo D'Oro, Melanie S Vacchio, Allan M Weissman,

Combination of miR-520d-3p and EphA2 siRNA treatment shows enhanced EphA2 inhibition and antitumor efficiency in vitro. Combination of miR-520d-3p and.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

Volume 12, Issue 6, Pages (March 2002)

Effect of bevacizumab on the proliferation of A2780 cells.

Presentation transcript:

Phosphatase activity associated with Themis is due to its association with Shp1. Phosphatase activity associated with Themis is due to its association with Shp1. (A) Tyrosine phosphatase activity assay with immunoprecipitated Themis and immunoprecipitated Shp1 from Themis+/+ thymocytes treated with or without Shp1/2 inhibitors (200 μM for 30 min before assay). (B) Tyrosine phosphatase activity assay assessing kinetics of phosphatase activity associated with immunoprecipitated Themis with different times of stimulation using biotinylated antibody cross-linking with streptavidin in Themis+/+ and Themis−/− thymocytes. All of the time points were compared with time 0 (i.e., unstimulated sample) for the purpose of statistical tests. (C) Tyrosine phosphatase activity assay with immunoprecipitated Shp1 with different times of stimulation using biotinylated antibody cross-linking with streptavidin in Themis+/+ and Themis−/− thymocytes. All of the time points were compared with time 0 (i.e., unstimulated sample) for the purpose of statistical tests. (D) Western blot showing levels of Themis (Top), Shp1 (Middle), and p-Shp1 (Bottom) associated with immunoprecipitated Shp1 from Themis+/+ and Themis−/− thymocytes with different times of stimulation. IB, immunoblot. Raw values of p-Shp1 and Shp1 were calculated using LI-COR Odyssey software with the help of the background subtraction method. Data are representative of three independent experiments. Unpaired t test with mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. ns, not significant. Monika Mehta et al. PNAS 2018;115:48:E11331-E11340 ©2018 by National Academy of Sciences